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由Methylomonas sp.同时从废水中去除甲和高化物. 与可溶性甲单氧基酶一起使用

Yuxuan Sang¹, Qinqin Hao², Yuechao Zhang²

¹CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai Shandong 264003, PR China; National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.

Bioresource technology

|December 12, 2024

概括

这项研究强调了Methylomonas sp. HYX-M1,一种有氧甲类植物,可以有效地去除废水中的甲和酸盐. 它独特的基因构成使其能够在高化物条件下生存和运作,为控制甲排放提供了一种新的策略.

科学领域:

环境微生物学环境微生物学
生物技术是生物技术.
废水处理废水处理

背景情况:

有氧甲类植物是控制废水处理中的甲排放的关键.
高酸盐水平抑制甲氧化和去除,对处理过程构成挑战.

研究的目的:

为了研究甲氧化和酸盐去除能力的甲基蒙娜斯 sp. 在高化物条件下HYX-M1.
为了阐明基因机制,该菌株的耐受性和功能在富含亚酸盐的环境下.

主要方法:

隔离和表征的甲基罗蒙纳斯 sp. 这就是HYX-M1.1.
在不同度的亚酸盐下测量甲氧化和亚酸盐去除速度.
对甲单氧化酶,酸盐减少酶和应激反应基因 (pmoA, mmoX, nirB, nirD, sod, ahpC) 的基因表达分析.

主要成果:

梅西洛蒙纳斯 (Methylomonas sp.) 是一种植物. 即使在6毫米的化物中,HYX-M1也证明了高效的甲氧化 (0.05毫摩尔/日) 和化物去除 (0.53毫米/日).
亚酸盐的去除主要归因于同化 (94.6-96.06%) 和脱 (3.10-5.03%).
观察到酸盐还原酶基因 (nirB,nirD) 和应激反应基因 (sod,ahpC) 的升级,与高酸盐耐受性相关.

结论:

梅西洛蒙纳斯 (Methylomonas sp.) 是一种植物. HYX-M1具有强大的甲氧化和酸盐去除能力,适用于高酸盐废水环境.
该菌株的遗传适应,包括增强的酸盐还原酶和应激反应基因表达,对其生存和功能至关重要.
这项研究提供了一个有前途的策略,用于应用有氧甲类植物,以减轻在具有挑战性的废水处理场景中的甲排放.

关键词:

同化同化同化同化甲氧化过程中的甲.梅西洛蒙纳斯 (Methylomonas sp.) 是一种植物.亚酸盐还原酶基因的基因亚酸盐的去除亚酸盐的去除

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