增强葡萄酒的劣乳性发酵:酵母人蛋白对Oenococcus oeni菌株的可变作用
Paloma Toraño1, Alba Martín-García1, Albert Bordons1
1Universitat Rovira i Virgili, Grup de Biotecnologia Enològica, Departament de Bioquímica i Biotecnologia, Facultat d'Enologia, C/ Marcel·lí Domingo 1, 43007 Tarragona, Catalonia, Spain.
Food microbiology
|December 12, 2024
概括
酵母人蛋白可以通过增强Oenococcus oeni的生存来改善乳发酵 (MLF). 这项研究表明,曼蛋白的影响因菌株而异,影响MLF动力学和酶活性,从而提高葡萄酒质量.
科学领域:
- 葡萄酒学 葡萄酒学 葡萄酒学
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 乳酸细菌 (LAB),特别是Oenococcus oeni,对于造葡萄酒中的乳发酵 (MLF) 是至关重要的.
- MLF将L-酸转化为L-乳酸,影响葡萄酒的风味和稳定性.
- 已知酵母人蛋白在具有挑战性的葡萄酒环境中支持LAB的生存.
研究的目的:
- 调查商用曼诺蛋白提取物对MLF动态的影响.
- 为了评估Oenococcus oeni对曼诺蛋白添加的菌株特定反应.
- 评估曼诺蛋白在MLF期间对α-曼诺酶活性的影响.
主要方法:
- 在模型条件下测试曼诺蛋白提取物对MLF的影响.
- 在十种O. oeni菌株中分析发酵动力学.
- 测量全细胞和超的α-曼诺酶活性.
主要成果:
- 观察到菌株特定的发酵动力学和人蛋白利用率.
- 基于菌株和度的曼诺蛋白添加调节的MLF结果.
- α-曼诺酶活性在超级生物中较高,并且在菌株之间有显著的差异.
结论:
- 曼诺蛋白与O. oeni的相互作用取决于菌株,影响MLF的效率.
- 了解这些相互作用是优化MLF和葡萄酒质量的关键.
- 建议在地下室条件下进行进一步的研究,以验证发现.
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