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相关概念视频

Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

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The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
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Nuclear Export of mRNA02:31

Nuclear Export of mRNA

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Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

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The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Types of RNA01:20

Types of RNA

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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
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Identification of Circular RNAs using RNA Sequencing
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循环RNA触发了无意义介导的mRNA衰变.

Sung Ho Boo1, Min-Kyung Shin2, Hyun Jung Hwang1

  • 1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea.

Molecular cell
|December 12, 2024
PubMed
概括

循环RNAs (circRNAs) 通过RNA-RNA相互作用触发mRNA降解,从而启动circRNA诱导的NMD (circNMD). 这种机制为向的mRNA降调疗法提供了潜力.

关键词:
欧洲司法委员会EJCRNA-RNA 相互作用灭症 (apoptosis) 是一种死亡的过程.背接拼接是一种背接拼接.环NMDMD 环NMD 环NMD 环圆形的RNARNA是一个圆形的RNA.在 eIF4A3A3 中.在 eIF4E 中,您可以使用 eIF4E.无意义介导的mRNA衰变是无意义介导的核帽结合复杂的核帽结合复杂的

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Use of Alu Element Containing Minigenes to Analyze Circular RNAs
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相关实验视频

Last Updated: Jun 5, 2025

Identification of Circular RNAs using RNA Sequencing
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Use of Alu Element Containing Minigenes to Analyze Circular RNAs
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Use of Alu Element Containing Minigenes to Analyze Circular RNAs

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科学领域:

  • 分子生物学分子生物学
  • 在RNA生物学,RNA生物学.
  • 基因规则 基因规则

背景情况:

  • 循环RNAs (circRNAs) 是RNA分子,在生物过程中具有调节作用.
  • 控制circRNA功能的精确分子机制尚未完全理解.
  • 研究circRNA介导的基因调节对于理解细胞过程至关重要.

研究的目的:

  • 阐明circRNAs调节基因表达的分子机制.
  • 为了识别和描述circRNA诱导的无意义介导的mRNA衰变 (circNMD).
  • 探索 circNMD 对选择性mRNA下调调节的治疗潜力.

主要方法:

  • 在circRNAs和mRNA 3' UTRs之间进行RNA-RNA相互作用分析.
  • 对外结合复合体 (EJC) 定位及其在mRNA衰变中的作用的研究.
  • 转录组分析以确定 circNMD 的候选者.
  • 在细胞亡和外源表达研究中对circNMD的实验验证.

主要成果:

  • circRNAs通过与mRNA 3' UTRs的直接RNA-RNA相互作用来促进mRNA的快速降解.
  • 这种相互作用招募EJC,导致EJC依赖的无意义介导mRNA衰变 (NMD),称为circNMD.
  • 数百个潜在的circNMD目标通过转录基因分析被确定.
  • circNMD在细胞亡中的作用得到了验证,并且在异源表达时显著降低了目标mRNA水平.

结论:

  • circRNAs通过一种新的circNMD途径诱导mRNA降解.
  • 该circNMD机制涉及RNA-RNA相互作用和EJC介导的NMD.
  • circNMD提出了一个有前途的治疗策略,用于针对性的mRNA下调.