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系统性红斑狼中下调的YTHDF2表达与炎症性和中性粒细胞因子产生有关

Hongshuai Zhao^1,2, Mengfan Lan^1,3, Zikun Huang^1,4,5

¹Department of Clinical Laboratory, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, No.17, YongwaiZhengjie, Donghu District, Nanchang, 330006, Jiangxi, China.

Clinical rheumatology

|December 12, 2024

概括

在全身性红斑狼 (SLE) 患者中,中性粒细胞中YTHDF2的水平下降,与炎症标志物和TNF-α的增加相关. 这表明YTHDF2可能是SLE的潜在诊断和治疗点.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

N6-甲基氨酸 (m6A) 修饰越来越多地被认为是其在全身性红斑狼 (SLE) 发病过程中的作用.
一种m6A读者蛋白质YTHDF2正在研究其在SLE中的特定作用,特别是在中性粒细胞中.

研究的目的:

调查SLE患者中性粒细胞中YTHDF2的表达水平.
探索YTHDF2表达与SLE的临床特征和病变发生之间的关系.
为了检查YTHDF2和TNF-α在SLE中性粒细胞中的相关性.

主要方法:

使用RT-qPCR对61名SLE患者和48名健康对照 (HC) 中性粒细胞中的m6A调节剂 (写字器,擦拭器,阅读器) 和炎症因子 (TNF-α,白内素) mRNA进行量化.
通过西式涂抹测定YTHDF2蛋白水平的确定.
在SLE患者中,YTHDF2表达和临床/实验室参数之间的相关性分析.
用SLE血清对HC中性粒细胞进行体外刺激,以评估YTHDF2和TNF-α表达变化.

主要成果:

与HC相比,在SLE患者的中性粒细胞中观察到YTHDF2的mRNA和蛋白质水平显著降低.
中性粒细胞中YTHDF2mRNA表达的降低与几个炎症标志物相关,包括L%,LMR,WBC,N,N%,NLR,dNLR和SII.
在SLE中性粒细胞中发现TNF-α的mRNA表达增加,显示与YTHDF2表达的反相关性. 用SLE血清刺激的HC中性粒细胞显示YTHDF2减少和TNF-α表达增加.

结论:

周围血液中性粒细胞中的YTHDF2水平与SLE的发病有关.
这些发现表明YTHDF2可能作为SLE诊断的潜在生物标志物和新的治疗点.
中性粒细胞中YTHDF2降低和TNF-α增加之间的相互作用突出了SLE发展的潜在机制.

关键词:

N6 - - 甲基亚诺辛的使用.作为一个中性粒细胞.系统性红血性狼 (Systemic lupus erythematosusus) 是一种全身性狼.瘤死亡因子-α YT521-植物学领域2 2

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