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准备和流细胞计分析的人类非淋巴细胞组织DC准则

Diana Dudziak^1,2, Lukas Heger^2,3, William W Agace^4,5

¹Institute of Immunology, Jena University Hospital, Friedrich-Schiller-University, Jena, Germany.

European journal of immunology

|December 13, 2024

概括

本文提供了详细的方案,用于准备和分析树突细胞 (DC) 从人体组织使用流细胞计. 这些方法对于免疫学家研究DC在健康和疾病中的功能至关重要.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

树突细胞 (DCs) 是关键的免疫细胞,参与启动适应性免疫反应.
需要标准化的协议可靠的DC隔离和分析从各种各样的人体组织.

研究的目的:

介绍人类树突细胞的制备和流动细胞计分析的最新方案.
从各种人类非淋巴细胞组织和瘤中生成单细胞悬浮液.

主要方法:

从人类肺,皮肤,牙,肠道,瘤和瘤排水淋巴结生成单细胞悬浮的详细协议.
流细胞测量技术用于树突细胞的表型分析.

主要成果:

准备高质量的单细胞悬浮剂的既定方法,适用于树突细胞分析.
用于各种人体组织和淋巴细胞器官的验证协议.

结论:

这篇文章是基础和临床免疫学家必不可少的资源.
提供的协议促进了强大的树突细胞研究和临床应用.

关键词:

树突细胞是一种树突细胞.流动细胞计量是流动细胞计量的方法.非淋巴瘤组织的组织.瘤是一个瘤.瘤排水淋巴结的淋巴结.

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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...