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在Passiflora spp中,叶子异质细胞和花外蜜汁发育需要依赖年龄的miR156的适当活性

Jessica Ribeiro Soares¹, Kerly Jessenia Moncaleano Robledo¹, Vinicius Carius de Souza²

¹Departamento de Biologia Vegetal/Laboratório de Cultura de Tecidos Vegetais/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil.

The New phytologist

|December 13, 2024

概括

年龄取决于miR156水平,通过调节SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE (SPL) 转录因子,控制激情花花外花蜜 (EFN) 的发展,影响花蜜成分和相互作用.

科学领域:

植物发育生物学植物发育生物学
分子遗传学分子遗传学
生态生态学生态生态学

背景情况:

激情花花外花蜜 (EFN) 对于昆虫的相互关系至关重要,但潜在的依赖年龄的发育调节尚不清楚.
众所周知,microRNA156 (miR156) 途径针对SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE (SPL) 转录因子,可以调节植物的年龄和发育.

研究的目的:

研究miR156/SPL模块在调节激情花EFN发育和形态学的作用.
了解年龄线索如何影响叶子发育的EFN形成和花蜜成分.

主要方法:

在两个Passiflora物种中对叶子和EFN形态的比较分析.
转基因植物具有改变miR156活性的表型特征.
对年轻叶子原始的转录组分析.
分析了花蜜糖的特征和与植物的相互作用.

主要成果:

低miR156水平与叶子成熟和EFN发育相关.
操纵miR156影响了叶子异质细胞和EFN大小/丰度.
针对miR156的SPL参与调节叶子和EFN相关基因.
改变的miR156活动改变了花蜜糖概况和访问模式.

结论:

在miR156/SPL模块间接调节EFN的发展以年龄依赖的方式.
EFN的发育与激情花叶的异质细胞发育计划密切相关.
这项研究揭示了连接植物年龄,叶子形态和生态相互作用的分子机制.

关键词:

帕西花科 (Passifloraceae) 是一个植物.植物外的花蜜.异质母细胞形成的过程 miR156 是一个156的数字.植物阶段的变化.

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