Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

4.6K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

7.5K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
7.5K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

2
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
2
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

2
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
2
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Ultrafast and steady-state optical characterization of multilayer PdS<sub>2</sub>.

Nanoscale·2026
Same author

Building a cost-effective mechanochemical Raman system: improved spectral and time resolution for <i>in situ</i> reaction and rheology monitoring.

Physical chemistry chemical physics : PCCP·2025
Same author

Green and Scalable Preparation of Highly Conductive Alkali Metal-dhta Coordination Polymers.

Inorganic chemistry·2024
Same author

Disulfide-Containing Nitrosoarenes: Synthesis and Insights into Their Self-Polymerization on a Gold Surface.

Langmuir : the ACS journal of surfaces and colloids·2024
Same author

Application of the Knife-Edge Technique on Transition Metal Dichalcogenide Monolayers for Resolution Assessment of Nonlinear Microscopy Modalities.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2024
Same author

Short-Term l-arginine Treatment Mitigates Early Damage of Dermal Collagen Induced by Diabetes.

Bioengineering (Basel, Switzerland)·2024

相关实验视频

Updated: Jun 5, 2025

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.2K

开发结构化照明显微镜,使用通过模拟微镜制片方法获得的传输衍射格.

Aleksa Denčevski1, Jovana Z Jelić1, Ana Senkić2

  • 1Institute of Physics Belgrade, University of Belgrade, National Institute of the Republic of Serbia, Belgrade, Serbia.

Microscopy research and technique
|December 13, 2024
PubMed
概括
此摘要是机器生成的。

我们开发了一种定制的结构化照明显微镜 (SIM),使用一种新的衍射格子制造方法. 这种先进的显微镜实现了增强的分辨率,揭示了微细的细胞结构,如天体细胞中的维丁丝.

关键词:
衍射格是一种射格.解析度测量的分辨率测量结构化照明显微镜结构化照明显微镜超高分辨率的显微镜.

更多相关视频

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.7K
High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

6.9K

相关实验视频

Last Updated: Jun 5, 2025

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.2K
Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.7K
High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

6.9K

科学领域:

  • 光学显微镜的使用方法
  • 纳米技术 纳米技术
  • 生物物理学的生物物理.

背景情况:

  • 结构化照明显微镜 (SIM) 提供超高分辨率的能力.
  • 衍射网格是显微镜中的关键光学元件.
  • 为光学元件开发具有成本效益和可定制的制造方法至关重要.

研究的目的:

  • 开发一个定制的结构化照明显微镜 (SIM).
  • 使用模拟微电影方法制造定制传输衍射格子.
  • 评估开发的SIM系统的分辨率增强.

主要方法:

  • 通过模拟微电影制作制造传输衍射格子.
  • 在MoS2单层上使用光珠和刀刃技术进行分辨率评估.
  • 光标记天体细胞的成像,专注于维丁丝.

主要成果:

  • 成功制造了具有定制常数的坚固,具有成本效益的衍射网格.
  • 通过使用两个独立的方法,证明了SIM在光成像上的分辨率增强.
  • 获得的超高分辨率图像揭示了星球细胞中细微的维门细胞骨架结构.

结论:

  • 定制制造的SIM系统与定制衍射格实现了增强的分辨率.
  • 模拟微镜制造方法为制造光学元件提供了一种多功能方法.
  • 开发的SIM有效地可视化了传统显微镜无法看到的细微亚细胞结构.