相关实验视频

Updated: Jun 5, 2025

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
08:54

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Published on: November 5, 2020

12.8K

对电子DNA样本的qPCR和ddPCR机制的检测灵敏度和精度的量化

Gledis Guri1,2, Jessica Louise Ray3, Andrew Olaf Shelton4

  • 1Norwegian Institute of Marine Research Framsenteret Tromsø Norway.

Ecology and evolution
|December 13, 2024
PubMed
概括

滴滴数字PCR (ddPCR) 在环境DNA (eDNA) 检测方面显示出比定量PCR (qPCR) 更高的灵敏度和精度,特别是在低度下. 这项研究指导了使用ddPCR改进的水生物种监测方法.

关键词:
ddPCRR 在线播放我们的eDNADNA鱼类监测 监测鱼类的监测qPCRR 是一个很好的方法.量化精度 量化精度 量化精度灵敏度 灵敏度 灵敏度 灵敏度 灵敏度

更多相关视频

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR11:37

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Published on: June 11, 2016

17.6K
A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters12:14

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

Published on: March 9, 2016

9.8K

相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
56.9K