粘膜关联的不变T细胞的作用因子功能随着年龄的增长而改变,并由RORγt调节
Zhi Yang1, Banxin Luo2, Minhuan Li3
1Department of General Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.
Frontiers in immunology
|December 13, 2024
概括
衰老将粘膜相关的不变T细胞 (MAIT) 从MAIT17转变为MAIT1,影响细胞毒性分子的产生. 这种由RORγt影响的变化可能会影响结直肠癌的预后,并提供潜在的治疗点.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 衰老研究研究 衰老研究
背景情况:
- 粘膜关联不变T细胞 (MAIT) 是粘膜屏障中的关键的与生俱来的T细胞.
- 影响MAIT细胞转录组和功能的因素,特别是在衰老过程中,尚不清楚.
研究的目的:
- 研究老化对小鼠肠道MAIT细胞的转录和功能特征的影响.
- 为了比较老鼠肠道MAIT细胞基因表达与人类肠道MAIT细胞.
主要方法:
- 单细胞RNA测序 (scRNA-seq) 用于分析来自老鼠模型的肠道MAIT细胞.
- 进行了功能测试,以评估MAIT细胞的能力.
- scRNA-seq被扩展到人类肠道MAIT细胞进行比较分析.
主要成果:
- 衰老诱导了小鼠肠道MAIT细胞从MAIT17转变为MAIT1形状,改变了细胞毒性分子的产生.
- 人类肠道MAIT细胞分离为MAIT1和MAIT17子集,基因表达模式与老年小鼠相似.
- RORγt调节IFNγ和IL17的产生;减少的RORC和Il17A与较差的结直肠癌存活率相关.
结论:
- 衰老驱动功能MAIT1/MAIT17细胞转移,可能由RORγt.介导.
- 改变MAIT细胞活性可能会影响疾病预后,特别是在结直肠癌中.
- 这项研究确定了调节MAIT细胞功能在衰老和疾病中的潜在治疗点.
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