多细胞瘤球体的无脚手架的发展,具有结构的空间特征和代谢辐射轮
Shelby N Bess1, Gaven K Smart1, Matthew J Igoe1
1Department of Biomedical Engineering, University of Arkansas, Fayetteville, AR USA.
In vitro models
|December 13, 2024
概括
这项研究开发了一个3D癌症球形模型与巨细胞,使用定制图像分析揭示不同区域的独特结构和代谢变化,推进瘤生物学研究.
科学领域:
- 细胞生物学 细胞生物学
- 生物技术是生物技术.
- 癌症研究 癌症研究
背景情况:
- 传统的二维细胞培养不能准确地模仿体内细胞微环境.
- 三维 (3D) 培养系统为研究复杂的细胞相互作用提供了更具生理相关性的模型.
- 描述3D文化需要专门的和强大的分析方法.
研究的目的:
- 建立一个包含癌细胞和巨细胞的3D多细胞球形模型.
- 开发和演示一个定制的图像分析程序用于球形形状的表征.
- 在3D球形模型中评估区域结构和代谢异质性.
主要方法:
- 使用癌细胞和巨细胞构建3D多细胞球体.
- 使用光显微镜量化亡,缺氧和巨细胞极化标记物 (CD80/CD206).
- 采用多光子显微镜和光寿命成像 (FLIM) 进行代谢表征 (NADH寿命,A1/A2比率).
主要成果:
- 观察到明显的微区域差异:死核中显示出更高的M2巨细胞 (CD206),亡 (CC3) 和缺氧 (HIFs) 标志物.
- 增殖边缘表现出更高的M1巨细胞 (CD80) 和增殖 (Ki67) 标志物.
- 代谢分析显示,与生殖边缘相比,死核中的NADH平均寿命较低,A1/A2比较高.
结论:
- 3D多细胞球形模型为研究瘤生物学提供了可靠的平台.
- 该模型允许在不同的球状微区域内详细评估结构和代谢变化.
- 开发的图像分析方法使复杂的3D细胞模型能够全面表征.
更多相关视频
相关概念视频
The Tumor Microenvironment
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
Metastasis
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Cancer Cell Migration through Invadopodia
Invadosome is a broad category of cell surface structures with proteolytic activity that degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However, invadopodia can...


