优化微波辅助的水热提取从红副产品中提取pectin及其物理化学,结构和功能性质
Imed E Benmebarek1,2, Diego J Gonzalez-Serrano2, Fatemeh Aghababaei3
1Laboratory of Organic Synthesis, Modeling and Optimization of Chemical Processes, Department of Chemistry, Faculty of Sciences, Badji Mokhtar-Annaba University, BP 12, 23000 Annaba, Algeria.
Food chemistry: X
|December 13, 2024
概括
微波辅助的热水提取优化了从果皮中提取pectin的回收. 这种方法产生了具有高抗氧化特性的素,适用于功能性食品和药品.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生化学
- 化学工程是化学工程的重要组成部分.
背景情况:
- 素是一种有价值的多糖,在果皮中丰富.
- 传统的素提取方法可能是低效和环境负担.
研究的目的:
- 优化微波辅助水热提取 (MAHE) 来从普通皮 (TPP) 中恢复pectin.
- 描述MAHE提取的pectin的物理化学特性和功能活动.
主要方法:
- 响应表面方法 (RSM) 与盒子-Behnken设计 (BBD) 被用于优化.
- 优化的提取参数包括pH值,辐射时间和温度.
- 评估的pectin属性包括提取产量 (EY),银酸含量 (GAC),化程度 (DE),乳化活性和总含量 (TPC).
- 进行了光谱 (1H NMR,FTIR) 和晶体 (XRD) 分析.
主要成果:
- 最佳的MAHE条件确定为pH1.7,12分钟的辐射和109°C.
- 获得的pectin产量 (EY) 为30.4%,GAC为72.3%,DE为45.2%.
- MAHE 素表现出显著的抗氧化特性 (TPC: 41.2 mg GAE/g) 和良好的乳化活性 (57.65%).
- XRD和SEM证实了提取的pectin的粗表面和晶体结构.
结论:
- MAHE是一种有效的技术,可以从果皮中提取高质量的pectin.
- 提取的pectin具有理想的功能性质,特别是抗氧化活性.
- 来自MAHE的pectin显示出在食品和制药行业作为天然成分的潜力.
相关概念视频
01:18Detergent Purification of Membrane Proteins
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
01:29Downstream Processing
Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...