从未成熟的神经元释放的外体细胞通过microRNA-7a-5p调节成年神经干细胞的分化
Xiujian Sun1,2, Yexiang Chen1, Ying Zhang1
1Department of Neurobiology and Acupuncture Research, Key Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third Affiliated Hospital of Zhejiang Chinese Medical University, Binjiang, Hangzhou 310053, Zhejiang, China.
Stem cells (Dayton, Ohio)
|December 13, 2024
概括
神经外体调节成人海马神经发生. 在这些外体中,MicroRNA-7a-5p通过准TCf12促进神经干细胞分化和神经发生.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 干细胞研究 干细胞研究
背景情况:
- 来自海马牙状环的外体对神经干细胞 (NSC) 分化和成年海马神经生成至关重要.
- 了解控制NSC命运决定的分子机制对于再生医学和神经科学至关重要.
研究的目的:
- 研究未成熟神经元分泌的外体在促进NSC分化中的作用.
- 在这些外体内识别特定的微RNA,这些微RNA调节成人海马神经发生.
- 阐明涉及microRNA-7a-5p及其在NSC分化中的点的分子途径.
主要方法:
- 在实验室培养小鼠神经干细胞 (NSCs).
- 从神经元培养中对外体隔离和表征.
- 微RNA测序以识别差异表达的微RNA.
- 使用miRNA模仿剂,抑制剂和siRNA的功能增益和丧失研究.
- 使用TCF12敲击和过度表达验证基因相互作用.
主要成果:
- 来自不成熟神经元的外基因组在体外促进了小鼠NSC的神经元分化.
- 发现miR-7a-5p在分化神经元的外体中被下调.
- 调节miR-7a-5p水平直接影响了NSC神经元分化.
- 证实miR-7a-5p直接准TCf12,这是NSC差异化的关键转录因子.
- Tcf12敲击抑制了,而过度表达促进了NSC差异化.
结论:
- 神经外体在调节成人海马神经发生方面发挥着至关重要的作用.
- 神经外体内的miR-7a-5p含量对于确定成人海马神经发生的命运至关重要.
- miR-7a-5p直接准TCF12以调节成人海马神经发生,突出了一个新的调节途径.
更多相关视频
06:52Author Spotlight: Collecting Neural Stem and Progenitor Cells from Live Animals Using a Novel Brain Milking Protocol
Published on: February 9, 2024
1.9K
08:48Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects
Published on: February 17, 2016
9.2K
相关概念视频
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
01:14Maintenance of the ES Cell State
2.2K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.2K