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圆形MYH9/miR-133a-3p/CXCR4轴:一种在精子受精和胚胎发育中的新型调节网络

Qian Sun^1,2, Yanyu Li^1,3,4, Wen Yang²

¹Suzhou Medical College, Soochow University, Suzhou, 215123, China.

Molecular biomedicine

|December 13, 2024

概括

精子微RNA-133a-3p通过准circMYH9/miR-133a-3p/CXCR4轴,对体外受精 (IVF) 的成功产生负面影响. 增强circMYH9可能会改善试管婴儿结果和胚胎发育.

科学领域:

生殖生物学生殖生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

精子微RNA (miRNA) 在受精和早期胚胎发育中起着至关重要的作用.
了解精子介导受精背后的分子机制对于改善体外受精 (IVF) 成功率至关重要.

研究的目的:

为了研究精子miRNAs对IVF受精率 (FR) 的影响.
探索精子介导受精和胚胎发育的调节机制.
确定潜在的生物标志物和治疗目标,以提高试管婴儿的结果.

主要方法:

高通量测序以识别差异表达的精子miRNAs.
生物信息分析 (Starbase,GO,KEGG,PPI) 用于识别调节性circRNAs和mRNAs.
在体外测定使用GC-2 spd小鼠精子细胞系和体内小鼠模型.

主要成果:

miR-133a-3p在与低受精率 (FR) 和可用胚胎率 (AER) 相关的精子中显著上调.
确定circMYH9/miR-133a-3p/CXCR4轴是一个关键的监管网络.
通过调节miR-133a-3p/CXCR4轴,circMYH9的过度表达增强了试管婴儿成功和胚胎发育.

结论:

miR-133a-3p通过准circMYH9/miR-133a-3p/CXCR4轴来抑制精子功能,受精和胚胎发育.
该circMYH9/miR-133a-3p/CXCR4网络作为一个潜在的生物标志物用于受精潜力和胚胎质量.
针对这个网络提供了一个新的治疗策略,以改善试管婴儿的结果和解决不孕症.

关键词:

CXCR4CXCR4CXCR4CXCR4CXCR4CXCR4CXCR4CXCR4CXCR4CXCR4C 电路MYH9H9 的情况.胚胎发育的发展试管婴儿受精率在试管婴儿受精率精子精子精子在 miR-133a-3p 中.

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