关节动物中的正规烯合成酶:细胞内基因转移
Xinlu Chen1, John M Urban2,3, Jens Wurlitzer4
1Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996.
概括
以前在昆虫中未知的正规烯合成酶 (TPS) 基因是通过从非昆虫关节动物的水平基因转移发现的. 菌蚊中的这些TPS基因在雄性中高度表达,这表明它们在昆虫交流和进化中的新角色.
科学领域:
- 分子生物学分子生物学
- 进化生物学 进化生物学
- 昆虫学 昆虫学是一门学科.
背景情况:
- 昆虫利用 terpenoids 进行物种内部和物种间的交流.
- 在植物和微生物中常见的正规合成酶 (TPS) 以前在昆虫中未被表征,尽管一些昆虫拥有相关的酶.
研究的目的:
- 调查昆虫中正规的烯合成酶 (TPS) 基因的存在和进化起源.
- 描述昆虫TPS基因的酶活性和表达模式.
主要方法:
- 在361个昆虫基因组中对TPS基因进行全基因组选.
- 遗传学分析和蛋白质数据库搜索以确定进化关系.
- 对来自昆虫和相关节肢动物的选定TPS酶进行酶分析.
- 在模型昆虫Bradysia coprophila*中进行基因表达分析.
主要成果:
- 在五种真菌蚊子 (Sciaridae) 和非昆虫关节动物 (如春尾虫 (Collembola) 和虫 (Acariformes)) 中发现了TPS基因.
- 来自鱼,科伦博拉和鱼类的TPS酶表现出单烯,六烯和/或二烯合成酶活动.
- 遗传学分析表明,TPS基因是从类动物转移到类动物的.
- 在*Bradysia coprophila*中,所有五个TPS基因在成年男性中表达最高.
结论:
- 昆虫中存在正规的TPS基因,可能是通过从非昆虫关节动物的水平基因转移获得的.
- 这些基因可能有助于昆虫的新型类生物合成和通信策略,具有性别和阶段特定的角色.
- 这一发现突显了昆虫的代谢创新,这种创新是由细胞系间基因转移驱动的.
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