结肠粘膜蛋白质的结性脊髓炎与肠道炎症的蛋白质组
Miao Cheng1,2, Siqi Xiao1,2, Shaer Kayi1,2
1The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.
PloS one
|December 13, 2024
概括
在患有肠道炎症的结性脊髓炎 (AS) 患者中,NMRAL1蛋白的表达有差异. 降低NMRAL1水平降低了炎症性细胞因子,这表明它在AS病变发生过程中的作用以及作为治疗点的潜力.
科学领域:
- 胃肠病学 胃肠病学
- 免疫学 免疫学 免疫学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 性脊柱炎 (AS) 与微观肠道炎症有关.
- 鉴定AS病原体中的关键蛋白质对于向治疗至关重要.
研究的目的:
- 在AS患有肠道炎症的患者的结肠中识别差异表达蛋白 (DEPs).
- 研究NMRAL1蛋白在AS炎症性细胞因子调节中的作用.
主要方法:
- 从健康个体,只有肠道炎症的患者,只有AS的患者和有肠道炎症的AS患者的结肠粘膜蛋白质组分析.
- 质谱学 (timsTOF Pro) 用于识别DEP.
- 在体外研究使用RAW264.7巨细胞评估NMRAL1mRNA和NMRAL1siRNA转染后的炎症性细胞因子水平 (TNF-α,IL-1β,IL-17,IL-23).
主要成果:
- 确定了患者群体之间蛋白质表达的显著差异.
- NMRAL1被确定为一个关键的DEP,特别是在AS患者的肠道炎症.
- 在巨细胞中抑制NMRAL1显著降低了促炎性细胞因子水平.
结论:
- 在AS患有肠道炎症的患者中,NMRAL1是关键的差异表达蛋白.
- NMRAL1在调节炎性细胞因子产生方面发挥着重要作用.
- NMRAL1代表了AS和相关的肠道炎症的潜在治疗标.
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