基于超声波处理的大豆乳糖蛋白和氨酸之间的相互作用机制构建纳米输送系统:结构,物理化学稳定性和生物可访问性
Xinru Cao1, Jia Cao1, Tianhe Xu1
1College of Food Science, Northeast Agricultural University, Harbin 150030, China.
Ultrasonics sonochemistry
|December 13, 2024
概括
豆蛋白 (SWP) 纳米粒子有效封装奎尔丁 (Que),增强其稳定性和生物可用性. 超声波处理优化了SWP结构,以提供卓越的奎尔素和防止降解.
科学领域:
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
背景情况:
- 奎尔 (Que) 是一种有益的黄类化合物,稳定性和生物可用性不佳.
- 豆蛋白 (SWP) 作为一种天然的输送系统具有潜力.
- 开发有效的纳米输送系统对于提高营养素的生物可访问性至关重要.
研究的目的:
- 使用超声波处理构建和描述一个大豆乳清蛋白 (SWP) - 奎尔 (Que) 纳米输送系统.
- 研究超声波功率对SWP-Que相互作用,纳米粒子形成和稳定性的影响.
- 评估SWP-Que纳米配送系统的保护作用和生物可访问性增强.
主要方法:
- 对SWP和Que修饰的超声波处理.
- 纳米粒子的表征 (粒子大小, ζ-潜力,分散).
- 用于相互作用研究的光谱分析 (光,FTIR).
- 封装效率的确定.
- 在各种条件下对Que的稳定性和生物可访问性进行评估.
主要成果:
- 最佳的超声处理 (300-500 W) 产生了更小,更稳定的SWP-Que纳米粒子,具有更高的z-潜在.
- 鉴定出疏水性相互作用是SWP和Que之间的主要结合机制.
- 超声波诱导的SWP展开增强了结合亲和力,在400W时达到95.63%的封装效率.
- SWP-Que纳米颗粒显著地保护了Que免受热,紫外线和储存降解.
- 在模拟消化过程中观察到增强的Que生物可访问性.
结论:
- 超声波修饰的SWP纳米颗粒提供了一个有效的Quercetin输送系统.
- 纳米输送系统提高了奎尔塞丁的稳定性和生物可用性.
- 这种方法对开发功能性食品成分和营养保健品具有前景.
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