制备和评估口服胰岛素纳米囊输送系统
Meng Zhang1, Chunxin Wang1, Junqian Pan1
1Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
International journal of biological macromolecules
|December 13, 2024
概括
研究人员使用PLGA开发了口服胰岛素纳米囊,增强了吸收,并提供了超过10小时的持续血糖控制. 这种新的输送系统克服了与传统胰岛素注射和口服相关的挑战.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术 纳米技术
- 药理学 药理学是指药理学的学科.
背景情况:
- 传统的皮下胰岛素注射会引起压力和波动.
- 口服胰岛素的输送面临着挑战:吸收不良,生物可用性低,降解.
- 开发有效的口服胰岛素输送系统对于糖尿病管理至关重要.
研究的目的:
- 开发和描述口服胰岛素纳米囊输送系统 (Orl-Ins-NPs).
- 为了提高口服胰岛素的稳定性,粘附性和透性.
- 评估Orl-Ins-NPs的体内低血糖疗效.
主要方法:
- 使用聚乳酸-联合甘油酸 (PLGA) 封装胰岛素.
- 用奇托/聚乙烯胺进行阴离子修饰,以增强粘附和透.
- 用响应pH的共聚物进行表面涂层,以保护胃部.
- 纳米囊尺寸,封装效率和装载能力的表征.
- 在老鼠体内监测血糖水平.
主要成果:
- Orl-Ins-NPs实现了140.08nm的平均尺寸,54.3%的封装效率和2.2%的负载能力.
- 阴离子修饰改善了粘液的附着性和透性.
- 响应pH的涂层提供了100%的胃保护.
- Orl-Ins-NPs在老鼠中显示持续降低血糖10小时,与传统注射不同.
- 该系统表现出良好的物理化学稳定性和长期的体内低血糖效应.
结论:
- 口内NP提供了一种有前途的口服胰岛素输送方法.
- 开发的系统克服了口服蛋白质药物输送的关键挑战.
- 这项技术有可能用于口服胰岛素和其他基于蛋白质的治疗方法的临床应用.
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