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黑色素保护精子细胞免受DNA损伤和由阿特拉辛诱导的亡

Tian-Ning Yang¹, Ning-Ning Huang², Yu-Xiang Wang²

¹Joint International Research Laboratory of Animal Health and Animal Food Safety, College of Veterinary Medicine, Southwest University, Chongqing 400715, PR China; College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.

Pesticide biochemistry and physiology

|December 13, 2024

概括

亚特拉辛除草剂会损害男性精子细胞,但黑激素可以防止这种损伤. 黑色素减轻了精子细胞中的DNA损伤和亡,为男性不孕症提供了潜在的治疗策略.

科学领域:

生殖毒理学生殖毒理学
内分泌学在内分泌学.
细胞生物学细胞生物学

背景情况:

阿特拉 (ATZ) 是一种常见的除草剂,与生殖功能障碍有关.
黑激素 (MLT) 是一种具有抗氧化特性的激素,可以抵消ATZ毒性.
针对ATZ诱导的男性生殖器损伤,MLT的保护作用的确切机制尚未完全理解.

研究的目的:

研究ATZ对精子细胞发育的影响.
确定MLT在预防ATZ诱导的精子生成失败中的作用.
阐明ATZ毒性和MLT保护的基础细胞机制.

主要方法:

在体内研究,使用暴露于ATZ和/或MLT的青春期小鼠.
使用GC-1细胞培养物的体外研究,以评估ATZ和MLT对精子的影响.
对精子活力,运动性,蛋白质组成和细胞损伤标记物的分析.

主要成果:

在小鼠丸中,ATZ暴露降低了精子的活力,运动性和改变了蛋白质组成.
在体内和体外,ATZ诱导了精子生成细胞 (GC-1细胞) 的DNA损伤和亡.
MLT治疗显著缓解了ATZ诱导的DNA损伤和亡.

结论:

ATZ 破坏精子生成,导致DNA 损伤和亡,特别是在精子生成细胞中.
通过减少DNA损伤和亡,MLT有效地减轻ATZ诱导的男性生殖毒性.
这项研究表明,MLT是ATZ诱导的男性不孕症的潜在治疗剂.

关键词:

亚特拉辛 (Atrazine) 是一种可以造成的DNA损伤是DNA损伤.黑色素是什么黑色素是什么黑色素是什么尸体灭 (Necroptosis) 是一种死亡的过程.精子发生是精子生成.

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