相关实验视频
Updated: Aug 15, 2026
13:42
RNA Secondary Structure Prediction Using High-throughput SHAPE
Published on: May 31, 2013
概括
研究人员确定了5SRNA基因转录中的速度限制中间体. 这种复合体在化过程中形成,涉及转录因子 (TFIIIA,TFIIIC,TFIIIB) 和RNA聚合酶III,优化转录启动.
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 生物化学 生物化学
背景情况:
- 精确的5S核糖体RNA基因转录对于细胞功能至关重要.
- 了解RNA聚合酶III转录启动的动力学对于阐明基因调节机制至关重要.
研究的目的:
- 通过体外运动分析来解决5SRNA基因准确转录的速度限制事件.
- 描述一个预启动中间体,并确定转录因子组合的顺序.
主要方法:
- 从5S基因促进体转录的体外动力学分析.
- 转录因子和RNA聚合酶III与5SDNA在不同条件下 (温度,,ATP) 的化.
- 介质复合体形成的特征及其对转录启动率的影响.
主要成果:
- 在延长的潜伏期内形成一个温度依赖的,限制速度的中间复合体,需要和ATP.
- 这种中间体涉及到5SDNA上的TFIIIA,TFIIIC,TFIIIB和RNA聚合酶III的固态度.
- 这种复合体的形成消除了实现稳定状态转录率的滞后,并且与模板承诺的稳定复合体不同.
- TFIIIB,TFIIIA和TFIIIC可以在5S基因上稳定隔离.
结论:
- 在5S基因上的因子相互作用的顺序是TFIIIA,TFIIIC,TFIIIB,其次是RNA聚合酶III.
- 一种前启动中间体,与稳定复合体不同,显著影响转录动力学.
- 这些发现为5S基因促进体的III类转录启动提供了详细的动态模型.
相关概念视频
02:05Gene Evolution - Fast or Slow?
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
02:41Chromatin Structure Regulates pre-mRNA Processing
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
02:53Ribosomal RNA Synthesis
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
02:36Transfer RNA Synthesis
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
02:36Transfer RNA Synthesis
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
02:53Ribosomal RNA Synthesis
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...