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相关概念视频

Antigen Processing Pathways01:31

Antigen Processing Pathways

932
MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
932
Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

455
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
455
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

658
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
658
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

519
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
519

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相关实验视频

Updated: Jun 5, 2025

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
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Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

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一个通用系统,通过单一的可适应循环来准MHCII类抗原复合体.

Haotian Du1, Jingjia Liu2, Kevin M Jude3

  • 1Department of Chemistry, Stanford University, Stanford, CA, USA.

Nature biotechnology
|December 13, 2024
PubMed
概括

我们开发了TRACeR-II,这是一种新的方法,可以快速创建针对主要基因相容性复合物II类 (MHCII) 分子的类特异性结合剂. 这种方法为开发针对自身免疫性疾病的新疗法提供了一个多功能平台.

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Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin
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Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
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Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

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相关实验视频

Last Updated: Jun 5, 2025

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
12:09

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

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科学领域:

  • 免疫学 免疫学 免疫学
  • 结构生物学 结构生物学
  • 蛋白质工程是指蛋白质的工程.

背景情况:

  • 主体组织相容性复合体II类 (MHCII) 分子向CD4+T细胞呈现抗原,对免疫反应至关重要.
  • 用T细胞受体 (TCR) 或TCR类抗体向-MHCII复合体显示了对自身免疫性疾病和微生物群耐受性的治疗潜力.

研究的目的:

  • 引入一种通用和快速的方法,TRACeR-II,用于开发特定的MHCII结合剂.
  • 创建具有小支架和单一识别循环的多功能结合剂,以准各种MHCII抗原.

主要方法:

  • 开发针对MHCII (TRACeR-II) 结合剂的抗原-MHC复合体记者有针对性的识别.
  • 使用一个小螺旋束脚手架,单一的识别循环用于-MHCII相互作用.
  • 采用计算式蛋白质设计,用于在基中生成特定的结合序列.

主要成果:

  • 证明了TRACeR-II结合剂的快速生成,其亲缘关系在低纳米到低微米范围.
  • 获得了与已确定的TCR和抗体可比的结合亲缘关系.
  • 从SARS-CoV-2序列计算成功设计了特定的结合序列.

结论:

  • TRACeR-II提供了一种简单而通用的方法来准抗原-MHCII复合体.
  • 这种方法绕过了对确定互补性的区域循环进行组合选择的需要.
  • TRACeR-II促进了针对免疫相关疾病的新疗法的开发.