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Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Updated: May 1, 2026

Universal Hand-held Three-dimensional Optoacoustic Imaging Probe for Deep Tissue Human Angiography and Functional Preclinical Studies in Real Time

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Published on: November 4, 2014

一个软件框架,用于功能光成像探头机械 (MechView) 的软件框架.

Sourav Halder¹, Wenjun Kou¹, Eric Goudie¹

¹Division of Gastroenterology and Hepatology, Department of Medicine, Feinberg School of Medicine, Kenneth C. Griffin Esophageal Center, Northwestern Medicine, Northwestern University, Chicago, Illinois, USA.

Neurogastroenterology and motility

|December 14, 2024

概括

一个新的网络框架量化了功能性光膜成像探针 (FLIP) 测量,用于诊断食道运动障碍. 该工具提供可靠的临床,机械和机器学习指标,提高诊断准确度.

关键词:

亚喀拉西亚亚喀拉西亚是一个亚洲地区.失足症是一种失足症.食道生物力学食道生物力学食道运动障碍食道运动障碍功能性光线成像探测器探测器

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Universal Hand-held Three-dimensional Optoacoustic Imaging Probe for Deep Tissue Human Angiography and Functional Preclinical Studies in Real Time

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科学领域:

胃肠病学胃肠病学
医疗器械医疗器械
计算生物学计算生物学

背景情况:

功能性膜成像探头 (FLIP) 对于诊断食道运动障碍至关重要.
目前存在的局限性是由于缺乏用于定量FLIP数据评估的软件.
食道壁的合规性估计是FLIP的一个关键应用.

研究的目的:

开发和验证一个软件框架,用于对FLIP测量进行全面的定量评估.
整合临床,机械和机器学习指标,以提高诊断能力.
为了解决在 FLIP 数据分析中对标准化软件的需求.

主要方法:

为统一的FLIP数据评估创建了一个基于Python的Web框架.
该框架计算了诸如食管胃结 (EGJ) 延伸度指数 (DI),最大EGJ开口,收缩力和位移体积等指标.
机器学习算法,包括虚拟疾病景观 (VDL) 和EGJ阻塞概率,在121名受试者中被纳入并验证.

主要成果:

该框架估计的临床指标与专家临床医生的诊断密切相关.
最少的错误分类发生,主要是在相邻的诊断类别之间.
虚拟疾病景观 (VDL) 概率指标显示了类似的准确性,临床医生批准的验证错误分类.

结论:

一个经过验证的FLIP网络框架可靠地估计了食道运动障碍诊断的各种指标.
该软件集成了临床,机械和机器学习方法,以提高诊断精度.
该框架为FLIP测量的定量分析提供了一个强大的解决方案.