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相关概念视频

RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: Jun 5, 2025

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
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A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

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功能性RNA采矿使用随机高通量选.

Li-Hua Liu1, Jinde Chen1, Shijing Lai1

  • 1Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd., Tongchaunghui South District, No. 40, Shangchong South, Haizhu District, Guangzhou, Guangdong 510000, P.R. China.

Nucleic acids research
|December 14, 2024
PubMed
概括
此摘要是机器生成的。

一种新的随机高通量选 (rHTS) 方法有效地识别功能性RNA分子. 这项技术发现了影响细菌生长和辅酶转换的新型RNA片段,展示了其对RNA采矿的力量.

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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
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相关实验视频

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物技术是生物技术.

背景情况:

  • 功能性RNA分子在细胞过程中起着至关重要的作用.
  • 目前选功能性RNA的方法有限.
  • 开发新的查技术对于推进RNA研究至关重要.

研究的目的:

  • 开发和验证一种用于识别功能性RNA的新型随机高通量查 (rHTS) 技术.
  • 应用rHTS用于发现参与细菌生长和辅酶代谢的RNA分子.

主要方法:

  • 合成一个大,统一的随机RNA片段 (~250-nt) 的图书馆.
  • 使用自循环 ribozyme 进行 RNA 片段的循环化,以提高稳定性.
  • 应用rHTS选影响大肠杆菌生长的RNA.
  • 在依赖辅酶的平台上使用rHTS识别调节RNA的NADPH/NADP+转换.

主要成果:

  • 确定了三个RNA片段显著促进大肠杆菌的生长,其中一个具有编码潜力.
  • 发现了两种非编码RNAs (ncRNAs) 在体内抑制大肠杆菌生长.
  • 确定了两种促进NADPH转化为NADP+的ncRNA.
  • 证明糖醇脱酶活性增加了18.3%至21.8%和23.2%,鉴定了ncRNAs的外源表达.

结论:

  • 开发的随机高通量查 (rHTS) 技术是功能性RNA发现的强大工具.
  • rHTS能够识别具有重要的生物功能的新型ncRNA.
  • 这项技术在各种领域具有广泛的应用,包括合成生物学和代谢工程.