重复的禁食事件使增强剂,转录因子活性和基因表达敏感,以支持增强生成
Noga Korenfeld1, Meital Charni-Natan1, Justine Bruse2
1Institute of Biochemistry, Food Science and Nutrition. The Robert H. Smith Faculty of Agriculture, Food and Environment. The Hebrew University of Jerusalem. 229 Herzl Street, Rehovot 7610001, Israel.
Nucleic acids research
|December 14, 2024
概括
重复的禁食时间重复的禁食时间
科学领域:
- 代谢调节 代谢调节 代谢调节
- 转录控制 转录控制
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 哺乳动物利用肝脏的葡萄糖和体的生产来忍受长时间的禁食.
- 禁食反应主要由转录机制调节.
- 了解反复禁食如何影响基因表达和代谢适应是至关重要的.
研究的目的:
- 在反复的禁食期间调查增强剂动态.
- 为了确定反复的禁食是否会引起与单次禁食相比不同的转录反应.
- 阐明基底适应反复的禁食信号的分子机制.
主要方法:
- 在小鼠中使用了替代性禁食 (ADF) 模型.
- 分析了基因表达变化和肝脏增强剂活性.
- 评估了PPARα与肝脏增强剂的结合.
- 野生型和肝细胞特异性PPARα缺乏的小鼠的比较反应.
主要成果:
- 替代性禁食 (ADF) 使数百个生成基因对随后的禁食发作敏感.
- 控制生成基因的肝增强剂在ADF后显示PPARα结合增加.
- 在野生类型的小鼠中,ADF增加了生成,而不是与单次禁食相比.
- 肝细胞特异性PPARα缺乏会取消ADF后观察到的增强生成.
结论:
- 肝细胞"记住"过去的禁食事件,增强它们对随后的禁食的转录反应.
- 由PPARα介导的肝细胞增强剂敏感化,在反复的禁食后增加了生成.
- 这项研究揭示了转录记忆在对反复的禁食信号的代谢适应中的机制.
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