Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.7K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.7K
Homologous Recombination02:31

Homologous Recombination

50.2K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.2K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

12.2K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
12.2K
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

9.8K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
9.8K
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

9.0K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.0K
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

7.0K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Mitochondrial dysfunction underlies cardiac contractility and growth defects in a zebrafish model of <i>NAA15</i>-related heart disease.

bioRxiv : the preprint server for biology·2026
Same author

The Uptake of Preregistration, and Its Impact on the Proportion of Supported Hypotheses, in Sports Science.

Sports medicine (Auckland, N.Z.)·2026
Same author

Targeting FGFR signaling overcomes therapeutic resistance and immune evasion in oncogenic PIK3CA-driven serous-like endometrial cancer.

Nature communications·2026
Same author

A conserved hormonal signalling-H2A.Z axis rapidly reorganizes 3D chromatin interactions in adipocyte thermogenesis.

Nature metabolism·2026
Same author

A dual role of EZH2 in regulating A-to-I RNA editing and mRNA stability through ADAR.

Nature communications·2026
Same author

Recovery From Heart Failure: Microvascular Mechanisms.

Circulation·2026

相关实验视频

Updated: Jun 5, 2025

Author Spotlight: Unveiling the Role of SNF2L in Replication Fork Stability and Genome Duplication
05:55

Author Spotlight: Unveiling the Role of SNF2L in Replication Fork Stability and Genome Duplication

Published on: August 23, 2024

439

在DNA断裂时,RPA和Rad27限制模板和倒置插入.

Yang Yu1, Xin Wang2,3,4, Jordan Fox1

  • 1Baylor College of Medicine, Department of Molecular and Human Genetics, One Baylor Plaza, Houston, TX 77030, USA.

Nucleic acids research
|December 14, 2024
PubMed
概括
此摘要是机器生成的。

在DNA双链断裂 (DSB) 上的模板插入在癌症中很常见. 这项研究揭示了一种使用微同学学的折叠机制,涉及DNA聚合酶三角和末端连接通路,由RPA缺乏症加剧.

更多相关视频

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

1.8K
Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

3.9K

相关实验视频

Last Updated: Jun 5, 2025

Author Spotlight: Unveiling the Role of SNF2L in Replication Fork Stability and Genome Duplication
05:55

Author Spotlight: Unveiling the Role of SNF2L in Replication Fork Stability and Genome Duplication

Published on: August 23, 2024

439
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

1.8K
Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

3.9K

科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个
  • 癌症研究 癌症研究

背景情况:

  • 在癌细胞中经常观察到DNA双链断裂 (DSB) 中形成模板插入.
  • 控制这些模板插入的精确机制和酶作用者仍然在很大程度上未被描述.

研究的目的:

  • 调查酵母中的DSBs模板插入背后的机制.
  • 确定与模板插入形成相关的酶要求和基因组特征.

主要方法:

  • 在DSB诱导后,利用安普利康序列分析酵母中修复的位点.
  • 研究了关键酶和DNA修复途径的作用,包括DNA聚合酶三角酶,非同类末端连接 (NHEJ),替代末端连接 (Alt-EJ) 和RPA.
  • 研究了影响RPA水平/功能和切除机器 (Sgs1, Exo1) 的突变对插入形成的影响.

主要成果:

  • 在DSB中识别了非常短的 (∼5-34 bp) 模板反向重复,通过利用微同质的折叠机制形成的DSB.
  • 证明了一种混合酶机制,涉及Polδ介导合成和NHEJ或Alt-EJ.
  • 在缺乏RPA或切除的突变体中观察到模板插入的增加,包括从遥远的基因组位置和脆弱位置的插入.
  • 发现了常见的复杂插入,涉及来自相同位置的两个序列的反向方向,表明微同学介导的模板切换.

结论:

  • 缺乏RPA,这种情况经常在癌细胞中发现,可能会促进模板插入的形成.
  • 这些发现阐明了一种通过模板插入DSBs来产生基因组不稳定的新机制.
  • 表明微同学介导的模板切换是形成这些插入的关键过程.