相关实验视频
Updated: Jun 5, 2025

Mitochondrial Respiration Quantification in Yeast Whole Cells
Published on: November 8, 2024
辅酶Q缺乏对萨基酵母酵母中乙醇发酵的影响
Ikuhisa Nishida1, Shogo Nishihara2, Tomohiro Kaino2
1Sakeology Center, Niigata University, Ikarashi, Niigata, Japan.
辅酶Q (CoQ) 影响了萨克酵母中的乙醇发酵. 含CoQ较少的酵母早期产生了更多的乙醇,而添加CoQ的酵母则产生了较少的乙醇.
科学领域:
- 生物化学 生物化学
- 微生物学 微生物学
- 酵母发酵 酵母发酵的方法
背景情况:
- 辅酶Q (CoQ) 对于通过有氧呼吸产生ATP至关重要.
- CoQ在乙醇发酵中的作用,特别是在糖酵母中,尚不清楚.
研究的目的:
- 调查辅酶Q水平与萨克酵母中乙醇生产率之间的关系.
- 为了确定CoQ缺乏或补充如何影响早期发酵.
主要方法:
- 使用了具有修改的辅酶Q水平 (缺乏和补充) 的萨克酵母菌株.
- 在发酵的早期阶段,监测了乙醇生产率.
主要成果:
- 辅酶Q的缺乏导致乙醇生产率大幅增加.
- 添加辅酶Q导致早期发酵期间乙醇生产率下降.
结论:
- 辅酶Q在萨克酵母的乙醇发酵过程中起着调节作用.
- 操纵辅酶Q水平可以改变早期发酵阶段的乙醇产量.
更多相关视频
14:53Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
07:38Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism
Published on: September 30, 2018
相关概念视频
Fates of Pyruvate
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
Fermentation
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Alcohols from Carbonyl Compounds: Reduction
Catalytic hydrogenation is similar to the reduction of an alkene or alkyne by adding H2 across the pi bond in the presence of transition metal catalysts like Raney Ni, Pd–C, Pt, or Ru. Aldehydes and ketones can be reduced by this method, often under mild to moderate heat (25–100°C) and...
Microbial Fermentation
Loss of Carboxy Group as CO2: Decarboxylation of β-Ketoacids
Preparation of Alcohols via Addition Reactions
The acid-catalyzed addition of water to the double bond of alkenes is a large-scale industrial method used to synthesize low-molecular-weight alcohols. An acidic atmosphere is required to allow the hydrogen in the water molecule to act as an electrophile and attack the double bond in an alkene. The addition of a proton to the double bond creates a carbocation intermediate. The proton preferentially bonds to the less substituted end of the double bond to create a more stable carbocation...