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Updated: Aug 11, 2026

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
15:28

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通过质量细胞计量对人类呼吸道的真菌菌菌特异性髓状细胞的表型和量化方案

Agano Kiravu1, Virgine Rozot1, Lauren Cruywagen1

  • 1South African Tuberculosis Vaccine Initiative, Institute of Infectious Disease and Molecular Medicine and Division of Immunology, Department of Pathology, University of Cape Town, Cape Town 7925, South Africa.

STAR protocols
|December 14, 2024
PubMed
概括

这项研究提出了一种新方案,用于分析人类呼吸道髓状细胞对菌根细菌抗原的反应,使用质细胞计. 该方法在冷后确保稳定的标记物测量,使免疫反应的灵活批量分析成为可能.

关键词:
细胞培养培养的细胞培养.免疫学 免疫学 免疫学质量细胞计量 (Mass Cytometry) 是一种测量质量细胞的方法.

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Microbial Growth Measurement: Direct Methods

Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
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Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...