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微量元素推动了人类病原菌Trichophyton rubrum中分泌的蛋白酶表达的上调
Oliver Blechert1, Shanyu Lan2, Shuzhen Xiong2
1Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China.
概括
微量元素显著提高了Trichophyton rubrum中分泌的蛋白酶的表达,这是导致皮肤感染的真菌. 这一发现可能解释了为什么某些皮肤疾病会增加感染的严重程度.
科学领域:
- 菌类学 菌类学是指菌类学.
- 分子生物学分子生物学
- 皮肤病学 皮肤病学
背景情况:
- 特里科菲顿 (Trichophyton rubrum) 是一种常见的真菌病原体,影响人体质化组织.
- 这种真菌通过分泌蛋白酶来侵入皮肤,这些蛋白质会分解宿主蛋白质.
- 从宿主获得营养的可用性对于真菌生长和入侵至关重要.
研究的目的:
- 研究微量元素和葡萄糖对T. rubrum中分泌蛋白酶的基因表达的影响.
- 为了确定由这些营养因素调节的特定蛋白酶基因.
主要方法:
- 用RNA测序 (RNAseq) 来分析基因表达.
- 这项研究在三个条件下比较了基因表达特征:质介质,含微量元素的质介质和含葡萄糖的质介质.
- 分析了31个分泌的蛋白酶基因.
主要成果:
- 将微量元素添加到氨酸介质中,使总体分泌蛋白酶表达从1.8%增加到3.3%.
- 诸如SUB4,MEP1,MEP3,MEP5,MEP9,LAP1,LAP2和MCPA等基因的表达显著更高.
- MEP5 (大约6倍) 和SUB4 (大约5.8倍) 是最高调节的基因.
结论:
- 微量元素在调节T. rubrum中分泌的蛋白酶的表达方面发挥着重要作用.
- 在像牛皮和亚托邦皮肤炎这样的疾病中,营养免疫力受到干扰可能会增加对严重T. rubrum感染的易感性.
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