聚糖 Polygonatum sibiricum可通过PI3K/Akt/mTOR信号通路改善骨肌肉衰老和线粒体功能障碍
Yang Li1, Zhongyuan Liu2, Hongyu Yan2
1Department of Orthopedic Surgery, Hubei Provincial Hospital of Traditional Chinese Medicine, Hubei Shizhen Laboratory, Affiliated Hospital of Hubei University of Chinese Medicine, Wuhan 430061, China.
概括
聚糖 (PSP) 通过激活PI3K/Akt/mTOR通路来对抗肌肉衰老和功能障碍. 这项研究强调了PSP的重点.
科学领域:
- 老年学和肌肉生理学
- 药理学和分子生物学
- 线粒体生物学 线粒体生物学
背景情况:
- 肉类症对老年人群构成重大健康风险.
- 聚糖 (PSP) 显示出抗氧化和抗炎性质.
- 通过PI3K/Akt/mTOR途径对骨肌肉衰老,肌细胞分化和线粒体功能产生PSP的特定影响仍然未被探索.
研究的目的:
- 研究PSP对肌肉衰老,肌细胞分化和线粒体功能障碍的影响.
- 阐明潜在的机制,特别是PI3K/Akt/mTOR信号通路的作用.
主要方法:
- 使用UHPLC-MS/MS.进行Polygonatum sibiricum的化学分析.
- 网络药理学,以确定PSP和肉症之间的共同目标和途径.
- 使用C2C12核细胞的体外研究来评估对衰老,分化和线粒体损伤的影响.
- 在体内研究使用D-银糖诱导的老化小鼠模型来评估骨肌肉的健康状况.
主要成果:
- 网络药理学确定了278个与线粒体功能和PI3K/Akt/mTOR通路相关的常见标.
- 在体外,PSP增强了C2C12细胞活力和肌管分化,调节了关键的衰老和肌肉特异性蛋白质.
- 通过上调PI3K/Akt/mTOR酸化,PSP提高了线粒体膜潜力,并降低了ROS水平.
- 在体内,PSP显著改善了老年小鼠的肌肉力量,耐力,质量和横截面积.
结论:
- 通过PI3K/Akt/mTOR通路激活,PSP有效地改善了骨肌肉衰老,缩和线粒体功能障碍.
- 这项研究为PSP对萨科佩尼亚的治疗潜力提供了新的见解.
- PSP代表了一种有前途的替代治疗剂,用于管理与年龄相关的肌肉衰退.
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