MabSelect VH3 蛋白 A 亲和树脂有效地分离含有不同数量的 VH3 域的抗体物种,并显示出改善的聚合物分离能力
Wanyuan Dong1, Rongrong Wang1, Yifeng Li1
1Downstream Process Development (DSPD), WuXi Biologics, 31 Yiwei Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China.
Protein expression and purification
|December 14, 2024
概括
新的MabSelect VH3蛋白A树脂选择性地结合VH3域,使副产品和聚合物的有效分离成为可能. 这与标准的蛋白质A树脂相比,为生物制品提供了更好的净化.
科学领域:
- 生物技术是生物技术.
- 蛋白质染色学 蛋白质染色学
- 生物工艺工程 生物工艺工程
背景情况:
- 蛋白A染色体是单克隆抗体净化的标准方法.
- 现有的蛋白质A树脂在分离特定的副产品和聚合物方面可能存在局限性.
研究的目的:
- 为了评估新型 MabSelect VH3 蛋白质 A 树脂的性能.
- 评估其基于VH3域号分离物种的能力,并改善聚合物去除.
主要方法:
- 使用了MabSelect VH3树脂进行染色分离.
- 将其性能与普通蛋白质A树脂进行了比较.
- 评估了具有不同VH3域数量和总量水平的物种分离.
主要成果:
- MabSelect VH3有效地分离了在VH3域数量上有所不同的物种.
- 与标准的蛋白质A树脂相比,证明了聚合物的更好的分离.
- 展示了副产品和聚合物的同时去除.
结论:
- MabSelect VH3为VH3域提供独特的选择性,增强净化策略.
- 在处理VH3变异副产品和高聚合物负载时,它是普通蛋白质A树脂的优质替代品,用于产品捕获.
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