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血微RNA与COVID-19严重程度和结果的关联

Sohair Salem¹, Randa Lotfy¹, Noha Eltaweel²

¹Molecular Genetics & Enzymology Department, Human Genetics and Genome Research Institute, National Research Centre, Cairo, Egypt.

Journal, genetic engineering & biotechnology

|December 14, 2024

概括

微RNAs (miRNAs) 在COVID-19患者中发生变化,作为疾病严重程度和患者结果的潜在生物标志物. 这项研究确定了COVID-19中失调的特定miRNAs,与疾病进展和预后相关.

科学领域:

生物化学生物化学
分子生物学分子生物学
传染性疾病传染性疾病

背景情况:

循环的microRNAs (miRNAs) 是对SARS-CoV-2感染的关键宿主反应.
miRNAs显示出作为COVID-19的诊断和预后生物标志物的潜力.

研究的目的:

调查miRNAs在COVID-19病原和严重性中的作用.
为了识别COVID-19患者中失调的特定miRNAs.

主要方法:

一项涉及120名COVID-19患者的病例控制研究,涉及严重程度和30名健康对照.
使用miRCURY LNA miRNA PCR面板和定量实时PCR分析了等离子体miRNA表达.

主要成果:

在COVID-19患者中,有11种特定的miRNAs (例如miR-375,miR-99b-5p) 显著过度表达.
对ROC曲线的分析表明了诊断潜力,AUC的综合值为0.93.
特定的miRNA表达模式与疾病严重程度和患者结果相关,其中五种miRNA可能调节COVID-19相关基因.

结论:

微RNA失调是COVID-19的一个标志.
改变的miRNA水平与COVID-19的严重程度和患者的预后有关.
已识别的miRNA可以作为COVID-19管理的有价值的生物标志物.

关键词:

在 COVID-19 疫情中,结果结果的结果.严重程度严重程度微RNAs 是一个微型RNA.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...