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鲁比斯科在一些针叶树和白动物中的酶和定量特性

Sakiko Sugawara¹, Kana Ito¹, Shin-Ichi Miyazawa²

¹Graduate School of Arts and Sciences, Iwate University, Morioka, Japan.

Journal of plant research

|December 14, 2024

概括

卢比斯科是一种关键的光合作用酶,与作物相比,在早期的陆地植物,如针叶树和白动物中,显示出较低的二氧化碳亲和力. 它们的酶丰富度与运动性质没有直接相关,这表明进化的权衡.

科学领域:

植物生理学植物生理学
生物化学生物化学
进化生物学进化生物学

背景情况:

鲁比斯科 (ribulose-1,5-bisphosphate carboxylase/oxygenase) 的动态特性对于光合作用至关重要,在早期进化中的陆地植物中尚不清楚.
鲁比斯科的效率影响了植物的碳固定和适应大气二氧化碳水平的变化.

研究的目的:

研究早期陆地植物 (针叶树,白) 中鲁比斯科的碳氧酶活性和丰度,并与C3作物进行比较.
评估Rubisco在这些植物群中的二氧化碳亲和度和催化周转率 (kcatc).

主要方法:

在五棵针叶树,两种白动物和三种C3作物中测量了Rubisco碳氧酶活性 (和CO2下的kcatc,不和CO2下的vcu).
基于vcu与kcatc的比率预测Rubisco的二氧化碳亲和力.
分析了与Rubisco的动力特性相关的分配.

主要成果:

在针叶树和白树中,Rubisco的周转率 (kcatc) 与C3作物相当.
鲁比斯科预测的二氧化碳亲和力在针叶树和白动物中通常低于C3作物.
针叶树和白树中的Rubisco kcatc比它们的二氧化碳亲和力预测的要低,分配也比预期的要低.

结论:

在早期的陆地植物 (针叶树,白) 中,鲁比斯科在催化率和二氧化碳亲和力方面都不是动态优越的.
鲁比斯科的丰富程度并不一定与它在这些植物中的动力特性直接相关.
在早期陆地植物中观察到的Rubisco特征可能反映了分子进化中的进化约束和适应.

关键词:

碳氧酶酶的活性活动.松树是一棵树.柳类动物 (Lycopods) 是一种流类动物.N个分配N个分配植物进化植物进化鲁比斯科 (Rubisco) 是一种古老的葡萄酒.

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