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Updated: Jun 5, 2025

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
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A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

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使用基于光显微镜的方法比较相对蛋白质-脂质体结合亲和力的协议.

Kar Ling Hoh1, Dan Zhang1

  • 1Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604, Singapore, Singapore; Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.

STAR protocols
|December 15, 2024
PubMed
概括

这项研究引入了一种光显微镜方法来量化蛋白质-脂质体结合亲和力,克服了对沉蛋白质的离心试验的局限性. 新协议可以适应各种蛋白质类型和系统.

关键词:
抗体是一种抗体.细胞生物学 细胞生物学显微镜 显微镜是指使用显微镜.蛋白质表达和净化过程

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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