YTHDF2通过降解人类角质细胞中的PIDD1mRNA促进诱导的恶性表型
Qian Zhang1, Jin Man1, Tianhe Zhao1
1Department of Environmental and Occupational Health, Sichuan University West China School of Public Health and West China Fourth Hospital, Chengdu, Sichuan, 610041, China.
Chemico-biological interactions
|December 15, 2024
概括
暴露于会增加YTHDF2蛋白,从而降解PIDD1mRNA. 这一过程抑制了亡并促进了恶性细胞的生长,揭示了诱导的皮肤癌的一个关键机制.
科学领域:
- 毒理学 毒理学 毒理学
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 是一种具有不完全理解机制的环境致癌物.
- N6-甲基氨酸 (m6A) 结合蛋白YTHDF2通过降解m6A修饰的mRNA来调节基因表达.
- 在致癌过程中YTHDF2的特定位在很大程度上是未知的.
研究的目的:
- 调查YTHDF2在诱导的致癌过程中的作用.
- 为了识别涉及致癌的YTHDF2的m6A修饰的目标mRNA.
主要方法:
- 人类角质细胞HaCaT细胞被暴露在酸中50代,以建立的致癌模型 (HaCaT-T).
- 在HaCaT和HaCaT-T细胞中分析了YTHDF2蛋白水平和m6A修饰状态.
- 研究了YTHDF2与PIDD1mRNA的结合及其对PIDD1mRNA降解和亡的影响.
主要成果:
- 在HaCaT-T细胞中,YTHDF2蛋白水平升高,其敲击抑制了恶性表型.
- 在HaCaT-T细胞中,m6A水平增加,m6A调节者的表达发生变化.
- YTHDF2以m6A-依赖的方式直接与PIDD1mRNA结合,促进其降解并减少酶-2-依赖的亡.
结论:
- YTHDF2在人类角质细胞的诱导的恶性表型中起着至关重要的作用.
- 由m6A介导的YTHDF2/PIDD1mRNA相互作用抑制了细胞灭绝并促进了细胞恶性瘤.
- 这项研究揭示了引起的皮肤癌的一个新机制,突出了YTHDF2作为潜在的治疗标.
更多相关视频
09:37A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
Published on: July 14, 2016
8.2K
![Chemical-Induced Skin Carcinogenesis Model Using Dimethylbenz[a]Anthracene and 12-O-Tetradecanoyl Phorbol-13-Acetate DMBA-TPA](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F60445.jpg&w=3840&q=50)
04:12Chemical-Induced Skin Carcinogenesis Model Using Dimethylbenz[a]Anthracene and 12-O-Tetradecanoyl Phorbol-13-Acetate DMBA-TPA
Published on: December 19, 2019
14.2K
相关概念视频
02:23Abnormal Proliferation
4.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
01:46Electron Transport Chain: Complex I and II
11.3K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
11.3K
01:31Translation
141.5K
Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of...
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of...
141.5K