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在小蜂巢甲虫,Aethina tumida Murray中鉴定出由法斯马病毒衍生的基因组序列和内源性病毒元素

Emily D Vu¹, Sijun Liu², Bryony C Bonning¹

¹Department of Entomology and Nematology, University of Florida, Gainesville, FL 32611, United States; University of Florida Genetics Institute, Gainesville, FL 32610, United States.

Journal of invertebrate pathology

|December 15, 2024

概括

小蜂巢甲虫 (SHB) 是一种入侵性害虫,它是新型病毒的息地. 研究人员在SHB中发现了一种新的phasmavirus,为对抗这种蜜蜂威胁的生物控制策略提供了潜力.

科学领域:

* 病毒学病毒学
* 昆虫学昆虫学
* 蜜蜂养殖蜜蜂养殖蜜蜂养殖

背景情况:

* 小蜂巢甲虫 (Aethina tumida Murray) 是一种侵袭性害虫,会影响蜜蜂群落.
* 它与蜜蜂的寄生关系和病毒传播中的潜在作用尚不清楚.
* 描述小蜂巢甲虫的病毒组对于理解其影响和探索生物控制至关重要.

研究的目的:

*为了描述小蜂巢甲虫 (SHB) 的病毒组.
* 为了识别与SHB相关的病毒,用于潜在的生物控制应用.
* 调查SHB在蜜蜂生态系统中的病毒传播中的作用.

主要方法:

*来自不同地理位置 (美国和南非) 的SHB的转录组和小RNA测序.
* 采集成体和幼虫SHB,包括一个SHB衍生细胞系 (BCIRL-AtumEN-1129).
* 生物信息分析包括转录组装和BLASTx注释.

主要成果:

*确定了来自南非的SHB和野外捕获的美国甲虫中可谓的新型肺炎病毒 (序列:Elliovirales) 几乎完整的序列.
*检测所有样本中的SHB基因组中集成的不同类型的马病毒的部分糖蛋白序列的转录.
*从综合病毒序列中获得的PIWI相互作用RNA的鉴定.

结论:

* 小蜂巢甲虫携带多种病毒组,包括一种新型的phasmavirus.
* 病毒序列,包括集成的法斯马病毒糖蛋白,在SHB中始终存在.
*需要进一步的研究来确定新型phasmavirus是否在小蜂巢甲虫中积极复制.

关键词:

一个ethina tumidaida的时间.内源性病毒元素是内源性病毒元素.斯马病毒的病毒.小蜂巢甲虫是一种小蜂巢甲虫.发现病毒序列的发现.

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