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在老鼠膀中硫化诱导的反应中与年龄和尿相关的变化
Fatma Aydinoglu1, Tugba Toyran2, Nuran Ogulener3
1Department of Pharmacology, Pharmacy Faculty, Cukurova University, Adana, Turkey.
Journal of physiological investigation
|December 15, 2024
概括
衰老改变了膀功能,硫化 (H2S) 起到了关键作用. 这项研究揭示了年龄和尿如何影响H 2 S介导的膀放松和收缩,这表明了与年龄相关的膀疾病的新治疗点.
科学领域:
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
- 生理学 生理学 生理学
- 生物化学 生物化学
背景情况:
- 衰老与膀功能的显著改变有关.
- 硫化 (H2S) 是一种在膀中合成的气态神经递质,与调节膀光滑肌肉度有关.
- 在膀功能中衰老,尿和H2S信号传递之间的相互作用仍然不完全理解.
研究的目的:
- 为了研究衰老和尿对小鼠膀组织中L-氨酸/H2S诱导的放松反应的影响.
- 检查完整和剥离的膀组织中卡巴霍尔诱导的收缩反应的与年龄相关的变化.
- 评估年轻人与老年膀组织中关键H 2 S合成酶的表达.
主要方法:
- 对年轻 (3-4个月) 和老年 (23-24个月) 鼠的膀组织进行比较分析.
- 对内源性 (L-氨酸) 和外源性 (硫化) H 2 S. 的放松反应的测量.
- 在完整的和尿脱皮的膀组织中对卡巴霍尔的收缩反应的评估.
- 免疫组合化学检测H2S合成酶 (cystathionine-β-synthase,cystathionine-γ-lyase,3-MST) 的作用.
主要成果:
- 在两年龄组的脱皮膀组织中,对L-氨酸/H2S的放松反应增加了.
- 与年轻膀相比,对卡巴霍尔的收缩反应在完整的老化膀中增加,但在剥离的老化膀中减少.
- 在老化膀组织的光滑肌肉中,CSE和3-MST的表达减少.
结论:
- 衰老和尿膜显著影响L-氨酸/H2S介导的膀放松和卡巴霍尔诱导的收缩.
- 与年龄相关的H2S和尿功能中的分子和生物变化可能会导致膀功能障碍.
- 这种L-氨酸/H2S途径对于与年龄相关的膀疾病,如过度活跃的膀和下泌尿道症状,具有潜在的治疗标.
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