α 甲诱导的生化和分子级联 基础的 绵羊卵巢粒粉细胞功能障碍 卵巢细胞功能障碍
Poonam Kumari Singh1, Bogapathi Sampath Kumar1, Sumanta Nandi1
1ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru, India.
Environmental toxicology
|December 16, 2024
概括
杀虫剂α-Cypermethrin (αCYP) 对绵羊卵巢颗粒细胞 (GCs) 的活力和功能产生负面影响. 它破坏了类固醇生成,改变了基因表达,对生殖健康构成风险.
科学领域:
- 生殖毒理学 生殖毒理学
- 内分泌学 在内分泌学.
- 细胞生物学 细胞生物学
背景情况:
- α-Cypermethrin (αCYP) 是印度广泛使用的杀虫剂.
- 暴露于杀虫剂可以破坏生殖功能.
- 绵羊卵巢颗粒细胞 (GCs) 对于卵泡发育和激素产生至关重要.
研究的目的:
- 调查α-Cypermethrin对绵羊GCs的影响.
- 评估αCYP对GCs生命力,生长,亡和类固醇生成的影响.
- 分析与类固醇激素合成相关的基因表达的变化.
主要方法:
- 绵羊GCs被培养在不同度的αCYP (0-100μM).
- 评估了细胞活力,代谢活性和细胞毒性标志物 (LDH,ROS,MDA).
- 分析了类固醇激素水平 (雌激素,孕激素) 和基因表达特征.
主要成果:
- αCYP从25μM起显著降低了GC的活力和代谢活性.
- 雌激素水平从1μM显著下降,而孕激素在10μM时增加.
- αCYP改变了参与类固醇发生和亡的关键基因的表达 (例如,CYP11A1,Star,BAX,CASP3,CYP17A1,ESR1).
结论:
- α-Cypermethrin对绵羊的GC产生有毒作用,损害它们的功能.
- αCYP 破坏了类固醇激素的产生,并改变了 GCs 中关键基因的表达.
- 这些发现突出了与牲畜中的αCYP暴露相关的潜在生殖风险.
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