关于使用CFD建模和实验验证的Yarrowia lipolytica高油密度生物反应器规模发酵的考虑
Sarah M Coleman1, Richard J Marx2, Morgan K Martinez1
1McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas, USA.
Biotechnology journal
|December 16, 2024
概括
这项研究探讨了使用废食用油的高油密度发酵,在实验室和试验规模上证明了成功的微生物生物转化. 计算流体动力学建模解决了工业应用的混合和质量转移挑战.
科学领域:
- 生物技术是生物技术.
- 化学工程是化学工程的重要组成部分.
- 微生物学 微生物学
背景情况:
- 废食用油是一种丰富,低成本的疏水原料,用于微生物生物转化.
- 目前的疏水基质发酵通常是小规模的或使用低基质度,限制了效率.
- 高油密度发酵提供了较低的体积要求,但存在混合和质量转移的挑战.
研究的目的:
- 调查使用废食用油的高油密度发酵的可行性和挑战.
- 使用计算流体动力学 (CFD) 在实验室和试点尺度上建模三相生物反应器.
- 以高度的废食用油进行发酵 (高达50%v/v).
主要方法:
- 3L和4000L生物反应器的计算流体动力学 (CFD) 建模.
- 使用Yarrowia lipolytica菌株L36DGA1.1.进行生物反应器发酵.
- 测试基板负荷从5%到50% (v/v).废食用油.
主要成果:
- CFD模型模拟了不同尺度的三相生物反应器中的混合和质量转移.
- 在高达50% (v/v) 的废食用油下取得了成功的发酵,这是文献中的高度.
- 该研究确定了扩大高油密度发酵的关键挑战和考虑因素.
结论:
- 使用废食用油进行高油密度发酵在实验室和试验规模上是可行的.
- 在这种发酵中,CFD建模是解决扩展挑战的宝贵工具.
- 这项研究支持有效的脂质衍生发酵产品的工业化实施.
相关概念视频
01:29Bioreactor Design and Operational System
Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
01:28Bioreactor Controls-I
Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...
01:18Bioreactor Controls-II
In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
01:22Bioreactor Controls-III
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
01:30Designing Growth Media for Bioreactors
Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...
01:14Scale-Up Processes
The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...