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Isolation of Leukocytes from the Human Maternal-fetal Interface
Published on: May 21, 2015
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怀孕涉及到母体循环中性粒细胞的代谢重新连接
Guillermina Calo1, Fátima Merech1, Florencia Sabbione2
1Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Universidad de Buenos Aires (UBA)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Journal of cellular physiology
|December 16, 2024
概括
怀孕改变了中性粒细胞的新陈代谢,增加了它们的功能和反应性氧物种 (ROS) 的产生. 热原体细胞影响中性粒细胞代谢,影响怀孕期间的免疫反应.
科学领域:
- 免疫代谢过程中的免疫代谢.
- 细胞的新陈代谢
- 免疫细胞功能 免疫细胞功能
背景情况:
- 怀孕需要精确的免疫和代谢调节,以实现母胎相互作用.
- 热细胞在支持胎儿的能量需求方面发挥着关键作用.
- 怀孕期间母亲中性粒细胞的代谢条件尚不清楚.
研究的目的:
- 调查热囊细胞衍生因素对非怀孕妇女中性粒细胞代谢重新连接的影响.
- 为了比较孕妇与非孕妇中性粒细胞的免疫代谢状态和功能.
- 探索新陈代谢在怀孕期间中性粒细胞功能中的作用.
主要方法:
- 来自非孕妇的中性粒细胞暴露于热囊细胞衍生因子.
- 分析中性粒细胞的功能,包括ROS的产生,NET的释放和迁移.
- 怀孕 (16-20周) 和非怀孕妇女中性粒细胞的比较.
- 评估代谢途径,包括葡萄糖吸收和脂肪酸氧化.
主要成果:
- 热囊细胞因子诱导了中性粒细胞的葡萄糖吸收和脂质积累,而没有增加ROS或NET释放.
- 来自热囊细胞的条件介质通过损害葡萄糖吸收,部分抑制了PMA诱导的NETosis.
- 孕妇的中性粒细胞显示基底ROS,葡萄糖吸收和脂质积累增加.
- 孕妇中性粒细胞中PMA诱导的NET释放量较高,部分依赖于脂肪酸氧化.
结论:
- 热囊细胞衍生因素有助于怀孕期间中性粒细胞的代谢概况.
- 免疫代谢机制对于在怀孕期间塑造中性粒细胞功能至关重要.
- 监测中性粒细胞代谢可能在评估怀孕状态方面具有临床潜力.
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