在中国多家医院的ST81 Clostridioides difficile的基因组流行病学
Xufen Xia1, Tao Lv2, Lisi Zheng2
1Department of Laboratory Medicine, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, People's Republic of China.
Infection and drug resistance
|December 16, 2024
概括
中国的Clostridioides difficile ST81菌株表现出高抗菌耐药性,分为两个独立的子系. 这凸显了加强监测的必要性,以监测传播并防止这种常见的C. difficile类型的疫情爆发.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 流行病学 流行病学
背景情况:
- Clostridioides difficile 序列类型 (ST) 81,通常与核型369相关,在中国普遍存在,其特征是TcdA阴性和TcdB阳性.
- 虽然ST81菌株对各种抗菌剂具有很高的耐药性,但详细的流行病学数据仍然有限.
- 了解C. difficile ST81的传播动态至关重要,因为它具有传播潜力.
研究的目的:
- 研究 Clostridioides difficile ST81.1. 的基因组流行病学和遗传特征.
- 分析C. difficile ST81菌株内的抗微生物耐药性基因和移动遗传元素.
- 识别不同的子系和它们的进化关系.
主要方法:
- 108种临床C. difficile ST81菌株的全基因组测序 (WGS),这些菌株从中国的五家三级医院 (2010-2021) 中分离出来.
- 多部位序列类型化 (MLST) 用于菌株鉴定和植物遗传分析.
- 识别抗菌素耐药性 (AMR) 基因和移动元件,包括转位子Tn916和Tn6189.
主要成果:
- 所有分析的C. difficile ST81基因组都是密切相关的,平均核心-基因组SNP距离为13.
- 基因组分析揭示了两个不同的,独立出现的子系 (SL I和SL II).
- 观察到AMR的高患病率,在SL I中主要发现特定的耐药性基因和TN916.
结论:
- 艰难的Clostridioides ST81代表了一个重要的克隆群体,具有两个独立的进化轨迹.
- 许多AMR基因和移动元件的存在表明ST81传播的风险很高.
- 强大的监测系统对于管理C. difficile ST81疫情和感染至关重要.
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