识别circRNA-miRNA-mRNA网络,以探索肺癌的潜在机制

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Updated: Jun 5, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Yajie Wang¹, Yu Fu¹

¹Medical School, Kunming University of Science & Technology, #727 Jing Ming Nan Road, Chenggong County, Kunming, 650500 Yunnan China.

Health information science and systems

|December 16, 2024

概括

这项研究揭示了肺癌 (LC) 中的新型循环RNA-microRNA-信使RNA网络. 五个关键基因被确定为肺腺癌的预后标志物,提供了新的治疗见解.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

循环RNAs (circRNAs) 在瘤发育中发挥作用,并且可以作为竞争的内源RNAs (ceRNAs) 起作用.
环RNA-microRNA-信使RNA (miRNA-mRNA) 调控网络在肺癌 (LC) 病原发生中的作用尚未完全理解.
研究这些网络对于理解LC和开发向治疗至关重要.

研究的目的:

在肺癌 (LC) 中探索新型circRNA-miRNA-mRNA ceRNA网络.
确定关键的调节基因和参与LC发展的途径.
发现肺腺癌 (LUAD) 的潜在预后生物标志物.

主要方法:

利用了来自基因表达大巴 (GEO) 的六个RNA表达数据集,用于circRNA,miRNA和mRNA.
使用Cytoscape构建了一个circRNA-miRNA-mRNA ceRNA网络,并确定了枢纽基因.
进行了蛋白质-蛋白质相互作用网络分析,基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析.

主要成果:

建立了一个全面的ceRNA网络,包括18个circRNA,32个miRNA和135个mRNA.
鉴定出了10个枢纽基因,包括VEGFA,FOS,MAD2L1,CREBBP,TYMS,EDN1,RFC5,KIF11,SLC2A1和TOP2A. 这些基因包括在内.
五个枢纽基因 (MAD2L1,TYMS,KIF11,SLC2A1,TOP2A) 与肺腺癌 (LUAD) 的预后有显著关联.

结论:

这项研究阐明了肺癌中的新型circRNA-miRNA-mRNA调节网络.
确定了关键的枢纽基因和通路,例如与氧气水平和核分裂相关的基因和通路,涉及LC.
这些发现为LC病原体和潜在的治疗策略提供了宝贵的见解,这些治疗策略针对预后生物标志物.

关键词:

肺癌是一种肺癌.病变的发生和发病.预测预后预测预测 ceRNARNA是什么意思环RNA 环RNA 是一个环RNA.

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lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...