血的预分析 (错误) 处理由H NMR代谢学研究
Daniel Malmodin1,2, Anders Bay Nord1, Huma Zafar3
1Swedish NMR Centre at the University of Gothenburg, SE-405 30 Gothenburg, Sweden.
ACS omega
|December 16, 2024
概括
预分析样本处理显著影响血成分,影响了代谢学研究. 这项研究开发了一种方法,以将血NMR资料中的预分析变化与真实生物数据分开.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
- 生物信息学是一种生物信息学.
背景情况:
- 分析前的样本处理 (采集,处理,储存) 极大地影响了血成分.
- 生物库样本往往缺乏详细的预分析信息,影响数据质量和研究解释.
- 代谢物度对预分析变化特别敏感,导致代谢学中的潜在误解.
研究的目的:
- 开发一种方法,以区分血中的预分析变异与生物信号.
- 通过删除分析前效应,创建一个数据空间,用于不偏见的代谢学分析.
- 识别和描述对血代谢物概况的预分析影响.
主要方法:
- 在等离子体核磁共振 (NMR) 数据上使用了对隐形结构直角投影差别分析 (OPLS-DA).
- 开发了一种多变量方法,将血NMR数据分成"原始样本空间"和"分析前处理空间".
- 研究的分析前效应,包括由甲醇沉和温度变化引起的效应.
主要成果:
- 成功地将血NMR数据分为不同的空间,代表生物变异和分析前处理.
- 已确认已知的分析前对葡萄糖,乳酸盐,甲素和酸盐等代谢物的已知影响.
- 观察到短链脂肪酸与甲醇沉期间的温度相关的可逆变化.
结论:
- OPLS-DA可以有效地建模和分离血代谢学中的生物信号的预分析变异.
- 这种方法可以为更准确的代谢学研究创建一个不那么偏见的"原始样本空间".
- 了解预分析效应对于可靠地解释生物银行样本数据和代谢学发现至关重要.
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