相关实验视频

Updated: May 3, 2026

A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice

A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice

Published on: March 23, 2016

运行-Mu-Ling颗粒通过抑制NLRP3 / GSDMD-介导的热致死途径来缓解与干眼相关的眼睛表面炎症损伤

Dan Luo¹, Hui-Jie Ji¹, Xue-Qing Yan¹

¹Department of ophthalmology, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing, People's Republic of China.

Journal of inflammation research

|December 16, 2024

概括

运行-Mu-Ling颗粒 (RMLG) 通过抑制NLRP3 / GSDMD介导的热,减少眼部炎症来治疗干眼. 这种传统的中医药显示出干眼治疗的前景.

科学领域:

眼科医生眼科眼科
免疫学免疫学免疫学
药理学药理学是指药理学的学科.

背景情况:

干眼 (DE) 是一种普遍影响眼表面健康的疾病.
底层DE病原和治疗的精确分子机制仍然不完全理解.
传统中国医学,如Run-Mu-Ling颗粒 (RMLG),用于DE,需要机械研究.

研究的目的:

阐明RMLG可以缓解DE中的眼表面炎症的分子机制.
调查热和NLRP3 / GSDMD信号通路在DE中的作用.
在DE模型中评估RMLG的治疗潜力.

主要方法:

已确立的体内大鼠和体内人类角膜上皮细胞系 (HCEC) 的DE模型.
评估角膜损伤,撕裂膜稳定性和HCEC活力.
利用免疫光学,西式涂抹和流动细胞计量来分析NLRP3 / GSDMD途径和烧死标记物.

主要成果:

RMLG显著改善了眼分泌和角膜完整性,同时增强了HCEC活动.
RMLG抑制了 pyroptosis,并抑制了NLRP3/GSDMD信号通路的激活.
观察到关键的炎症性细胞因子水平降低,包括IL-18,IL-1β和TNF-α.

结论:

通过NLRP3/GSDMD介导的热致死在DE的发病过程中起着至关重要的作用.
通过抑制这种炎症途径,RMLG通过抑制这种炎症途径发挥治疗作用.
RMLG代表了DE的潜在治疗剂,对理解其分子作用和临床应用有影响.

关键词:

在RMLG中使用RMLG.角膜损伤导致的角膜损伤眼睛干燥是指眼睛干燥.这是一种炎症炎症炎症炎症.热致灭 (pyroptosis) 是一种致的过程.传统的中国医药传统的中国医药.

更多相关视频

Induction of Ocular Surface Inflammation and Collection of Involved Tissues

Induction of Ocular Surface Inflammation and Collection of Involved Tissues

Published on: August 4, 2022

Establishment of A Mouse Model of Aqueous Deficiency Dry Eye

Establishment of A Mouse Model of Aqueous Deficiency Dry Eye

Published on: November 1, 2024

相关概念视频

Open Angle Glaucoma: Treatment

Open Angle Glaucoma: Treatment

In open-angle glaucoma, the iridocorneal angle remains open, but the trabecular meshwork becomes stiff, slowing down the outflow of aqueous humor. This causes a buildup of aqueous humor in the anterior chamber, leading to a sudden increase in intraocular pressure. The treatment for open-angle glaucoma focuses on reducing the elevated intraocular pressure by either decreasing the secretion of aqueous humor or increasing its outflow.
Drugs such as carbonic anhydrase inhibitors, α2- and...

Ophthalmic Drug Delivery Systems

Ophthalmic Drug Delivery Systems

Ophthalmic drug delivery faces major limitations due to poor absorption across the corneal membrane. This process is primarily driven by diffusion and is influenced by two main factors: the physicochemical properties of the drug and tear drainage. Most ophthalmic drugs, such as pilocarpine, epinephrine, atropine, and local anesthetics, are weak bases. They are typically formulated at an acidic pH to enhance chemical stability. However, this leads to high ionization, reducing their ability to...

Microbiome of the Eye

Microbiome of the Eye

The human eye has a specialized microbiota that reflects its unique anatomical and immunological environment. This low-biomass microbial community predominantly colonizes the conjunctiva and eyelid margins, playing a vital role in ocular surface homeostasis and defense. Despite its proximity to the richly colonized facial skin, the ocular surface maintains a distinct microbial profile due to continuous mechanical and biochemical defense mechanisms.The conjunctival surface hosts fewer microbial...