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血蛋白质组学用于在儿童结核病中发现新的生物标志物

Andrea Fossati^1,2,3, Peter Wambi⁴, Devan Jaganath^5,6

¹J. David Gladstone Institutes, San Francisco, 94158, California,USA.

medRxiv : the preprint server for health sciences

|December 16, 2024

概括

研究人员开发了一种新的血生物签名,以改善儿童结核病 (TB) 诊断. 这种非唾液生物标志物可以准确地识别儿科结核病,有可能加速治疗并挽救生命.

科学领域:

发现生物标志物的发现.
蛋白质组学是指蛋白质组学.
儿童传染病儿童传染病

背景情况:

结核病 (TB) 仍然是全球儿童死亡的主要原因.
目前儿童结核病的诊断方法往往不准确和侵入性.
对于儿童早期结核病检测而言,非常需要非唾液基生物标志物.

研究的目的:

开发一种基于血的生物签名,用于诊断儿童肺结核.
识别可表明儿科结核病的新型蛋白质生物标志物.
提高儿科结核病诊断的准确性和速度.

主要方法:

在511名儿童的血样本上使用高通量数据独立采集质谱法 (DIA-PASEF-MS).
分析了确诊结核病的儿童和非结核病呼吸道疾病的儿童的蛋白质组数据.
采用机器学习来导出预测性的生物签名.

主要成果:

在结核病和非结核病儿科组之间确定了47种不同调节的蛋白质.
开发了三种节的生物签名 (4-6种蛋白质),具有高诊断准确性 (AUC 0.86-0.88).
生物签名达到或超过了世界卫生组织针对结核病查测试的目标产品概况值.

结论:

开发了一种新的非吐血生物签名,用于儿科结核病诊断.
这种生物特征显示出有可能显著减少儿童的诊断延迟.
这些发现提供了对儿科结核病宿主反应的见解,也是全球疾病管理的工具.

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