规范的资源重新分配在转录上被硬连接到酵母的压力反应中
Rachel A Kocik1, Audrey P Gasch1,2
1Center for Genomic Science Innovation, University of Wisconsin-Madison, Madison, WI 53706.
bioRxiv : the preprint server for biology
|December 16, 2024
概括
在Saccharomyces cerevisiae中,一般化的压力反应涉及转向细胞资源. 抑制生长基因加快了防御机制,使人们能够更快地适应和耐受盐等压力因素.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 生物利用通用应激反应来生存不利条件,通常涉及从生长转向防御基因表达的转变.
- 在压力期间激活防御基因和抑制生长基因之间的相互作用是复杂的,并未完全理解.
研究的目的:
- 研究转录因子Msn2/Msn4和Dot6/Tod6在Saccharomyces cerevisiae对盐应激反应中的作用.
- 阐明这些因素协调抑制生长基因和激活防御基因的机制.
主要方法:
- 在Saccharomyces cerevisiae中利用了分子,基因组和单细胞微流体技术.
- 在盐应激条件下检查了转录因子Msn2,Msn4,Dot6和Tod6之间的遗传相互作用.
主要成果:
- 丢失Dot6/Tod6导致盐的适应速度较慢,而丢失Msn2/Msn4则导致压力期间的生长速度更快.
- 通过Dot6/Tod6暂时抑制生长基因被发现可以加速Msn2/Msn4介导的应激反应.
- 显示 Msn2/Msn4 调节 DOT6 mRNA 生产并影响 Dot6 激活,直接影响生长基因抑制.
结论:
- 增长促进基因的Msn2/Msn4介导的抑制对于有效的适应和获得耐压力至关重要.
- 转录因子 Msn2/Msn4 直接调节细胞资源的重新分配,这对于建立自身应激反应至关重要.
- 研究结果提供了对各种生物体普遍应激反应的保存机制的见解.
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