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Updated: Jun 5, 2025

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

在B组中的一张CRISPRi图书馆屏幕菌株识别了表面免疫原蛋白 (Sip) 作为多个宿主相互作用的调解者

K Firestone¹, K P Gopalakrishna², L M Rogers¹

¹Indiana University School of Medicine, Department of Medicine.

bioRxiv : the preprint server for biology

|December 16, 2024

概括

B组链球菌 (GBS) 表面蛋白Sip对于感染至关重要. 击倒Sip会增加炎症并损害GBS.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
遗传学遗传学是一个

背景情况:

乙组链球菌 (GBS) 是围产期感染的重要原因之一.
表面蛋白质是GBS与宿主相互作用的关键,但很难从遗传学上研究.
了解GBS表面蛋白的功能对于开发新疗法至关重要.

研究的目的:

为了识别参与宿主-病原体相互作用的GBS表面蛋白质.
调查GBS表面蛋白质在调节巨细胞因子反应中的作用.
探索GBS表面蛋白的治疗潜力.

主要方法:

对654个GBS基因组的生物信息分析确定了66个保存的表面蛋白质基因.
一个CRISPR干扰 (CRISPRi) 库被用来准这些基因在GBS.
使用类似于THP-1巨的细胞来评估细胞因子对GBS变异的反应 (TNF-α,IL-1β).

主要成果:

破坏表面免疫原蛋白 (Sip) 基因显著增加了IL-1β分泌.
西普在依赖卡斯巴-1的IL-1β调节中起作用.
在小鼠模型中,Δsip突变体显示生物膜形成受损,粘附性降低,子宫持久性降低.

结论:

GBS表面蛋白质Sip对于调节宿主免疫反应和病变发生至关重要.
Sip影响细菌的粘附性,生物膜的形成和持久性.
西普是新型GBS疗法和疫苗的潜在目标.

关键词:

这就是CRISPR干扰的原因.B组的链球菌.细胞因子反应的反应主体-病原体相互作用表面蛋白质表面蛋白质

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相关概念视频

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...