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相关概念视频

Downstream Processing01:29

Downstream Processing

Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...

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相关实验视频

Updated: Jun 23, 2026

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli
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Published on: November 30, 2018

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通过超过来净化循环RNA.

Karen Guillen-Cuevas, Xiaoming Lu, Marc R Birtwistle

    bioRxiv : the preprint server for biology
    |December 16, 2024
    PubMed
    概括
    此摘要是机器生成的。

    循环RNA (circRNA) 为治疗提供了增强的稳定性. 这项研究表明,超过有效净化circRNA,达到86%的纯度,超过了可扩展制造的传统方法.

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    相关实验视频

    Last Updated: Jun 23, 2026

    Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli
    10:38

    Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

    Published on: November 30, 2018

    9.6K
    Fecal micro RNA Isolation
    05:35

    Fecal micro RNA Isolation

    Published on: October 28, 2020

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    Quantification of Circular RNAs Using Digital Droplet PCR
    08:39

    Quantification of Circular RNAs Using Digital Droplet PCR

    Published on: September 16, 2022

    3.4K

    科学领域:

    • 生物技术是生物技术.
    • 分子生物学分子生物学
    • 生物化学 生物化学

    背景情况:

    • 使者RNA (mRNA) 疫苗激起了对mRNA疗法的兴趣.
    • 线性mRNA由于降解而寿命短,限制了其治疗潜力.
    • 循环RNA (circRNA) 提供了卓越的稳定性,但需要高纯度,这很难实现.

    研究的目的:

    • 探索超过方法,从体外转录 (IVT) 混合物中净化编码蛋白质的circRNA.
    • 评估超过的有效性与大小排除高性能液态染色学 (SE-HPLC) 相比.

    主要方法:

    • 使用聚硫膜 (30300 kDa MWCO) 进行超过的研究.
    • 测量了circRNA,线性前体RNA和切割的RNA对象的选系数.
    • 确定RNA净化临界流量和最佳操作条件.

    主要成果:

    • 通过超过实现了86%的纯度和>50%的产量.
    • 尺寸排除高性能液体染色学 (SE-HPLC) 获得了41%的纯度和45%的产量.
    • 超过在circRNA净化中表现出卓越的性能.

    结论:

    • 超过是一种高效的方法,用于净化研究规模的circRNA.
    • 超过的可扩展性表明它有可能用于大规模的circRNA治疗制造.
    • 这种净化方法解决了开发基于circRNA的稳定疗法的关键瓶.