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Updated: Jun 5, 2025

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR
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Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR

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使用SYBR®使用绿色实时PCR检测香团顶部病毒的敏感批量检测

Jay-Vee S Mendoza1, Fe M Dela Cueva1, Jen Daine L Nocum1

  • 1Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños, College, Laguna 4031 USA.

Virusdisease
|December 16, 2024
PubMed
概括

一种新的实时PCR方法可以有效地检测香群顶部病毒 (BBTV). 这种敏感且具有成本效益的方法有助于检疫部门管理这种破坏性香病.

关键词:
香束顶部病毒这就是DNA-RR.检测 检测 检测 检测 检测qPCRR 是一个很好的方法.

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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