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查和研究血液miRNAs作为皮质甲状腺癌的潜在诊断标记

Xize Li¹, Wen Qin², Wenting Wang¹

¹Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Cheeloo College of Medicine, Jinan, 250012, People's Republic of China.

OncoTargets and therapy

|December 16, 2024

概括

这项研究确定了针对乳头甲状腺癌 (PTC) 的新型基于血液的microRNA (miRNA) 生物标志物. miR-182-5p显示出作为诊断和预后指标的潜力,与PTC患者的不良结果相关.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
生物标志物发现发现

背景情况:

微RNAs (miRNAs) 是参与瘤发生的基因表达的关键调节者.
乳头甲状腺癌 (PTC) 的发展涉及复杂的遗传和调节性改变.
识别特定的分子标记对于准确的PTC诊断和预后至关重要.

研究的目的:

在PTC中调查miRNA-目标基因调控网络.
为了确定新的,基于血液的miRNA生物标志物用于PTC诊断和预后.
验证特定miRNAs在PTC启动和进展中的作用.

主要方法:

来自GEO数据库 (GSE50901,GSE113629) 的miRNA和基因表达数据的生物信息学分析.
利用GEO2R进行miRNA和PTC中的基因的差异表达分析.
使用GO和KEGG的预测miRNA-目标相互作用,途径和生物效应.
通过QRT-PCR对人体血液样本进行验证,并与现有的瘤标志物相关联.

主要成果:

在PTC组织中鉴定了2116个差异表达的miRNAs (1968年上调,148下调).
五种miRNAs (miR-221-3p,miR-222-3p,miR-182-5p,miR-135a-5p,miR-34a-5p) 与PTC预后有着强烈的联系.
在PTC患者中,miR-182-5p表达显著增加,与预后不佳正相关.

结论:

通过综合生物信息学和实验验证,确定了用于PTC的新型基于血液的miRNA生物标志物.
miR-182-5p成为PTC诊断和预后的一个有前途的生物标志物.
这些发现为通过可访问的生物标志物检测改善PTC患者管理提供了新的途径.

关键词:

生物信息学是一种生物信息学.血液样本血液样本不同表达的基因.这是一个微型RNA.皮囊甲状腺癌是甲状腺癌.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...