外在电场调节神经元发育,并增加视网膜器官中的光受体数量
Deepthi S Rajendran Nair1, Anika Gupta1, Ege Iseri2
1Department of Ophthalmology, USC Roski Eye Institute, University of Southern California, Los Angeles, CA, United States.
Frontiers in neuroscience
|December 16, 2024
概括
短时间接触电场可以增强视网膜器官中的神经元发育,促进光受体群. 优化电刺激可以改善有机体技术并减少化学品的使用.
科学领域:
- 神经科学是一个神经科学.
- 发育生物学 发展生物学
- 生物技术是生物技术.
背景情况:
- 内在和外在电场对于中枢神经系统的发育至关重要.
- 视网膜有机体是研究视网膜发育和疾病的有价值模型.
研究的目的:
- 研究短期外部电场暴露对人类视网膜有机体发育和分化的影响.
- 评估电刺激如何影响视网膜细胞群和成熟标志物.
主要方法:
- 人类胚胎干细胞衍生的视网膜器官在第80天被暴露在双相电场中60分钟.
- 免疫组织化学,定量聚合酶链反应 (qPCR) 和RNA测序被用来分析细胞发育和基因表达.
主要成果:
- 电刺激显著促进了神经元发育,增加了光受体种群.
- RNA测序揭示了杆光受体,米勒细胞,水平细胞和亚马克林细胞的基因的上调.
- 观察到视网膜色素表皮和视网膜细胞基因的下调,根据电场参数的变化.
结论:
- 外界电场在有机体中显著影响早期视网膜发育.
- 优化电场参数可以提高视网膜器官技术,并可能减少对化学处理的依赖.
相关概念视频
The Retina
The retina is a layer of nervous tissue at the back of the eye that transduces light into neural signals. This process, called phototransduction, is carried out by rod and cone photoreceptor cells in the back of the retina.
Vision
Vision is the result of light being detected and transduced into neural signals by the retina of the eye. This information is then further analyzed and interpreted by the brain. First, light enters the front of the eye and is focused by the cornea and lens onto the retina—a thin sheet of neural tissue lining the back of the eye. Because of refraction through the convex lens of the eye, images are projected onto the retina upside-down and reversed.
Anatomy of the Eyeball
The eye is a spherical, hollow structure composed of three tissue layers. The outer layer — the fibrous tunic, comprises the sclera — a white structure — and the cornea, which is transparent. The sclera encompasses some of the ocular surface, most of which is not visible. However, the 'white of the eye' is distinctively visible in humans compared to other species. The cornea, a clear covering at the front of the eye, enables light penetration. The eye's middle layer, the vascular tunic,...
Photoreceptors and Visual Pathways
At the molecular level, visual signals trigger transformations in photopigment molecules, resulting in changes in the photoreceptor cell's membrane potential. The photon's energy level is denoted by its wavelength, with each specific wavelength of visible light associated with a distinct color. The spectral range of visible light, classified as electromagnetic radiation, spans from 380 to 720 nm. Electromagnetic radiation wavelengths exceeding 720 nm fall under the infrared category, whereas...


