桃树木废物的利用用于Trichoderma stromaticum经济高效的粉酶生产:稳定性和工业潜力
Stephanie Alexandrina Gillet1, Murillo Nascimento Silva2, Elck Almeida Carvalho3
1Department of Biological Sciences, Universidade Estadual de Santa Cruz, Ilhéus, Bahia, Brazil.
Mycology
|December 16, 2024
概括
这项研究优化了Trichoderma stromaticum的粉酶产量,使用桃子棕废物. 通过特定的源实现了增强的酶活性,显示了工业应用的潜力.
科学领域:
- 生物技术是生物技术.
- 酶学 是一种酶学.
- 工业微生物学 工业微生物学
背景情况:
- 氨基酶是各种生物技术和工业过程中的关键酶.
- 农业工业废物为酶生产提供了可持续和低成本的基质.
- 皮菌 (Trichoderma stromaticum) 是一种具有酶合成潜力的真菌物种.
研究的目的:
- 通过使用农业工业废物,研究Trichoderma stromaticum的粉酶的产生.
- 通过战略性添加源来优化酶产量.
- 描述产生的氨酶的物理化学特性,使其适用于工业.
主要方法:
- 固态发酵 (SSF) 使用桃子棕废物作为唯一的基质.
- 优化源成分 (和酸) 使用简单的中心体混合物设计.
- 物理化学表征包括最佳pH值,温度,稳定性测试和金属离子效应.
主要成果:
- 桃树的棕废物 (29.2 U/gds) 的使用Trichoderma stromaticum产生了氨酶.
- 优化源 (1%:酸,7:3重/重) 增加了45% (42.4U/gds) 的氨酶活性.
- 氨酶在pH值4.0和50-60°C时表现出最佳活性,在各种pH值和温度范围内具有广泛的稳定性,并且在存在SDS和β-mercaptoethanol时具有最佳活性.
结论:
- 桃子棕废物是Trichoderma stromaticum生产氨酶的可行的低成本基质.
- 优化补充剂显著提高了酶产量.
- 特征性氨酶表现出强大的稳定性和活性,表明工业应用的巨大潜力,特别是在食品加工中.
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